Profiling gene transcription in vivo reveals adipose tissue as an immediate target of tumor necrosis factor-α: Implications for insulin resistance

Hong Ruan, Philip D G Miles, Christine Marie Ladd-Acosta, Kenneth Ross, Todd R. Golub, Jerrold M. Olefsky, Harvey F. Lodish

Research output: Contribution to journalArticle

Abstract

Despite extensive studies implicating tumor necrosis factor (TNF)-α as a contributing cause of insulin resistance, the mechanism(s) by which TNF-α alters energy metabolism in vivo and the tissue specificity of TNF-α action are unclear. Here, we investigated the effects of TNF-α infusion on gene expression and energy metabolism in adult rats. A 1-day TNF-α treatment decreased overall insulin sensitivity and caused a 70% increase (P = 0.005) in plasma levels of free fatty acids (FFAs) and a 46% decrease (P = 0.01) in ACRP30. A 4-day TNF-α infusion caused insulin resistance and significant elevation of plasma levels of FFAs and triglycerides and reduction of ACRP30. Plasma glucose concentration was not altered following TNF-α infusion for up to 4 days. As revealed by oligonucleotide microarrays, TNF-α evoked major and rapid changes in adipocyte gene expression, favoring FFA release and cytokine production, and fewer changes in liver gene expression, but favoring FFA and cholesterol synthesis and VLDL production. There was only a moderate repressive effect on skeletal muscle gene expression. We demonstrate that TNF-α antagonizes the actions of insulin, at least in part, through regulation of adipocyte gene expression including reduction in ACRP30 mRNA and induction of lipolysis resulting in increased plasma FFAs. TNF-α later alters systemic energy homeostasis that closely resembles the insulin resistance phenotype. Our data suggest that blockade of TNF-α action in adipose tissue may prevent TNF-α-induced insulin resistance in vivo.

Original languageEnglish (US)
Pages (from-to)3176-3188
Number of pages13
JournalDiabetes
Volume51
Issue number11
StatePublished - Nov 1 2002
Externally publishedYes

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Insulin Resistance
Adipose Tissue
Tumor Necrosis Factor-alpha
Genes
Nonesterified Fatty Acids
Gene Expression
Adipocytes
Energy Metabolism
VLDL Cholesterol
Organ Specificity
Lipolysis
Gene Expression Regulation
Oligonucleotide Array Sequence Analysis
Skeletal Muscle
Triglycerides
Homeostasis
Insulin
Cytokines
Phenotype
Glucose

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Profiling gene transcription in vivo reveals adipose tissue as an immediate target of tumor necrosis factor-α : Implications for insulin resistance. / Ruan, Hong; Miles, Philip D G; Ladd-Acosta, Christine Marie; Ross, Kenneth; Golub, Todd R.; Olefsky, Jerrold M.; Lodish, Harvey F.

In: Diabetes, Vol. 51, No. 11, 01.11.2002, p. 3176-3188.

Research output: Contribution to journalArticle

Ruan, Hong ; Miles, Philip D G ; Ladd-Acosta, Christine Marie ; Ross, Kenneth ; Golub, Todd R. ; Olefsky, Jerrold M. ; Lodish, Harvey F. / Profiling gene transcription in vivo reveals adipose tissue as an immediate target of tumor necrosis factor-α : Implications for insulin resistance. In: Diabetes. 2002 ; Vol. 51, No. 11. pp. 3176-3188.
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