The profiles of prostanoid biosynthesis from endogenous arachidonic acid in 16 established cell lines derived from 4 histological classes of human carcinomas were determined by capillary gas chromatography-mass spectrometry. Detectable quantities of prostanoids were isolated from the culture medium of cell lines representative of the different histological classes of human tumors: colorectal adenocarcinomas (one of three cell lines); ovarian adenocarcinomas (one of three cell lines); prostate adenocarcinomas (zero of two cell lines); and small cell carcinomas of the lung (zero of three cell lines). Prostaglandins E2 and F(2α) were the only prostanoids synthesized in detectable quantities. Prostaglandin E2 biosynthesis (mean ± SD, pmol/106 cells, n = 4) in cell lines exhibiting positive prostaglandin H synthase activity was: LoVo (colorectal adenocarcinoma, 0.4 ± 0.1); A2780 (ovarian adenocarcinoma, 1.3 ± 0.3); NCI-H322 (bronchioalveolar cell carcinoma, 8.4 ± 3.1); NCI-H358 (bronchioalveolar cell carcinoma, 7.8 ± 2.4); EKVX (adenocarcinoma of the lung, 21.3 ± 5.5); and A427 (large cell undifferentiated carcinoma of the lung, 12.6 ± 2.8). Prostaglandin F(2α) production (pmol/106 cells ± SD) was: LoVo (0.3 ± 0.1); NCI-H322 (0.6 ± 0.2); NCI-H358 (0.4 ± 0.1); EKVX (1.8 ± 0.4); and A427 (11.1 ± 3.1). These findings suggest that within certain limitations cultured tumor cells provide simplified experimental systems for determination of prostaglandin biosynthetic characteristics of human tumors and that prostanoid biosynthesis may be particularly characteristic of certain non-small cell carcinomas of the lung.
|Original language||English (US)|
|Number of pages||6|
|Publication status||Published - 1988|
ASJC Scopus subject areas
- Cancer Research