Proficiency testing of clinical microbiology laboratories using modified decontamination procedures for detection of nontuberculous mycobacteria in sputum samples from cystic fibrosis patients

S. Whittier, K. Olivier, P. Gilligan, M. Knowles, P. Della-Latta, J. Besser- Weik, P. Charache, M. Coyle, M. J. Ferraro, D. Gaskins, L. Heifits, R. Hopfer, S. Kehl, P. Kitterman, P. Newcomb-Gayman, E. Ng, C. Pierson, S. Reed, J. W. Rourke, F. SantorelliD. Stieritz, R. Verall, R. Wadowsky, T. Williams, M. York

Research output: Contribution to journalArticlepeer-review

Abstract

An improved decontamination method has been demonstrated to reduce overgrowth of mycobacterial media by Pseudomonas aeruginosa and allow the successful recovery of nontuberculous mycobacteria (NTM) from cystic fibrosis (CF) patients. Twenty microbiology laboratories participating in a multicenter investigation designed to determine the significance of NTM in CF patients were required to demonstrate proficiency in the incorporation of this improved method; this was accomplished by successful decontamination and culture workup of a panel of simulated sputum samples seeded with P. aeruginosa and various NTM. All laboratories successfully recovered NTM from samples with acid-fast bacillus (AFB) smear scores of 3+/4+ (i.e., 2 to 18 or >18 organisms/field). Low-inoculum samples (1+/2+ AFB smears [2 to 18 organisms in 100 or 10 fields]) were problematic in that processed specimens were often smear and/or culture negative.

Original languageEnglish (US)
Pages (from-to)2706-2708
Number of pages3
JournalJournal of clinical microbiology
Volume35
Issue number10
DOIs
StatePublished - 1997
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)

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