Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43

Hyun Kyoung Lee, Byoung Hee Lee, Seung Hyeok Seok, Min Won Baek, Hui Young Lee, Dong Jae Kim, Yi Rang Na, Kyoung Jin Noh, Sung Hoon Park, Dutta Noton Kumar, Hiroaki Kariwa, Mina Nakauchi, Suk Jin Heo, Jae Hak Park

Research output: Contribution to journalArticlepeer-review

Abstract

Severe acute respiratory syndrome (SARS) is a life-threatening disease for which accurate diagnosis is essential. Although many tools have been developed for the diagnosis of SARS, false-positive reactions in negative sera may occur because of cross-reactivity with other coronaviruses. We have raised polyclonal and monoclonal antibodies (Abs) using a recombinant form of the SARS virus nucleocapsid protein. Cross-reactivity of these anti-SARS Abs against human coronavirus (HCoV) 229E and HCoV OC43 were determined by Western blotting. The Abs produced reacted with recombinant SARS virus nucleocapsid protein, but not with HCoV 229E or HCoV OC43.

Original languageEnglish (US)
Pages (from-to)165-167
Number of pages3
JournalJournal of Veterinary Science
Volume11
Issue number1
DOIs
StatePublished - Mar 2010

Keywords

  • Cross-reactivity
  • HCoV 229E
  • HCoV OC43
  • Recombinant nucleocapsid protein
  • SARS

ASJC Scopus subject areas

  • veterinary(all)

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