Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43

Hyun Kyoung Lee; Byoung Hee Lee; Seung Hyeok Seok; Min Won Baek; Hui Young Lee; Dong Jae Kim; Yi Rang Na; Kyoung Jin Noh; Sung Hoon Park; Dutta Noton Kumar; Hiroaki Kariwa; Mina Nakauchi; Suk Jin Heo; Jae Hak Park

doi:10.4142/jvs.2010.11.2.165

Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43

Hyun Kyoung Lee, Byoung Hee Lee, Seung Hyeok Seok, Min Won Baek, Hui Young Lee, Dong Jae Kim, Yi Rang Na, Kyoung Jin Noh, Sung Hoon Park, Dutta Noton Kumar, Hiroaki Kariwa, Mina Nakauchi, Suk Jin Heo, Jae Hak Park

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Severe acute respiratory syndrome (SARS) is a life-threatening disease for which accurate diagnosis is essential. Although many tools have been developed for the diagnosis of SARS, false-positive reactions in negative sera may occur because of cross-reactivity with other coronaviruses. We have raised polyclonal and monoclonal antibodies (Abs) using a recombinant form of the SARS virus nucleocapsid protein. Cross-reactivity of these anti-SARS Abs against human coronavirus (HCoV) 229E and HCoV OC43 were determined by Western blotting. The Abs produced reacted with recombinant SARS virus nucleocapsid protein, but not with HCoV 229E or HCoV OC43.

Original language	English (US)
Pages (from-to)	165-167
Number of pages	3
Journal	Journal of Veterinary Science
Volume	11
Issue number	1
DOIs	https://doi.org/10.4142/jvs.2010.11.2.165
State	Published - Mar 2010
Externally published	Yes

Keywords

Cross-reactivity
HCoV 229E
HCoV OC43
Recombinant nucleocapsid protein
SARS

ASJC Scopus subject areas

veterinary(all)

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10.4142/jvs.2010.11.2.165

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Lee, H. K., Lee, B. H., Seok, S. H., Baek, M. W., Lee, H. Y., Kim, D. J., Na, Y. R., Noh, K. J., Park, S. H., Kumar, D. N., Kariwa, H., Nakauchi, M., Heo, S. J., & Park, J. H. (2010). Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43. Journal of Veterinary Science, 11(1), 165-167. https://doi.org/10.4142/jvs.2010.11.2.165

Lee, HK, Lee, BH, Seok, SH, Baek, MW, Lee, HY, Kim, DJ, Na, YR, Noh, KJ, Park, SH, Kumar, DN, Kariwa, H, Nakauchi, M, Heo, SJ & Park, JH 2010, 'Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43', Journal of Veterinary Science, vol. 11, no. 1, pp. 165-167. https://doi.org/10.4142/jvs.2010.11.2.165

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abstract = "Severe acute respiratory syndrome (SARS) is a life-threatening disease for which accurate diagnosis is essential. Although many tools have been developed for the diagnosis of SARS, false-positive reactions in negative sera may occur because of cross-reactivity with other coronaviruses. We have raised polyclonal and monoclonal antibodies (Abs) using a recombinant form of the SARS virus nucleocapsid protein. Cross-reactivity of these anti-SARS Abs against human coronavirus (HCoV) 229E and HCoV OC43 were determined by Western blotting. The Abs produced reacted with recombinant SARS virus nucleocapsid protein, but not with HCoV 229E or HCoV OC43.",

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doi = "10.4142/jvs.2010.11.2.165",

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Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43

Abstract

Keywords

ASJC Scopus subject areas

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