Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43

Hyun Kyoung Lee; Byoung Hee Lee; Seung Hyeok Seok; Min Won Baek; Hui Young Lee; Dong Jae Kim; Yi Rang Na; Kyoung Jin Noh; Sung Hoon Park; Dutta Noton Kumar; Hiroaki Kariwa; Mina Nakauchi; Suk Jin Heo; Jae Hak Park

doi:10.4142/jvs.2010.11.2.165

Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43

Hyun Kyoung Lee, Byoung Hee Lee, Seung Hyeok Seok, Min Won Baek, Hui Young Lee, Dong Jae Kim, Yi Rang Na, Kyoung Jin Noh, Sung Hoon Park, Dutta Noton Kumar, Hiroaki Kariwa, Mina Nakauchi, Suk Jin Heo, Jae Hak Park

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Severe acute respiratory syndrome (SARS) is a life-threatening disease for which accurate diagnosis is essential. Although many tools have been developed for the diagnosis of SARS, false-positive reactions in negative sera may occur because of cross-reactivity with other coronaviruses. We have raised polyclonal and monoclonal antibodies (Abs) using a recombinant form of the SARS virus nucleocapsid protein. Cross-reactivity of these anti-SARS Abs against human coronavirus (HCoV) 229E and HCoV OC43 were determined by Western blotting. The Abs produced reacted with recombinant SARS virus nucleocapsid protein, but not with HCoV 229E or HCoV OC43.

Original language	English (US)
Pages (from-to)	165-167
Number of pages	3
Journal	Journal of Veterinary Science
Volume	11
Issue number	1
DOIs	https://doi.org/10.4142/jvs.2010.11.2.165
State	Published - Mar 2010
Externally published	Yes

Keywords

Cross-reactivity
HCoV 229E
HCoV OC43
Recombinant nucleocapsid protein
SARS

ASJC Scopus subject areas

General Veterinary

Access to Document

10.4142/jvs.2010.11.2.165

Cite this

Lee, H. K., Lee, B. H., Seok, S. H., Baek, M. W., Lee, H. Y., Kim, D. J., Na, Y. R., Noh, K. J., Park, S. H., Kumar, D. N., Kariwa, H., Nakauchi, M., Heo, S. J., & Park, J. H. (2010). Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43. Journal of Veterinary Science, 11(1), 165-167. https://doi.org/10.4142/jvs.2010.11.2.165

Lee, HK, Lee, BH, Seok, SH, Baek, MW, Lee, HY, Kim, DJ, Na, YR, Noh, KJ, Park, SH, Kumar, DN, Kariwa, H, Nakauchi, M, Heo, SJ & Park, JH 2010, 'Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43', Journal of Veterinary Science, vol. 11, no. 1, pp. 165-167. https://doi.org/10.4142/jvs.2010.11.2.165

@article{de4c6a433ce541908d73ee82f7889125,

title = "Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43",

abstract = "Severe acute respiratory syndrome (SARS) is a life-threatening disease for which accurate diagnosis is essential. Although many tools have been developed for the diagnosis of SARS, false-positive reactions in negative sera may occur because of cross-reactivity with other coronaviruses. We have raised polyclonal and monoclonal antibodies (Abs) using a recombinant form of the SARS virus nucleocapsid protein. Cross-reactivity of these anti-SARS Abs against human coronavirus (HCoV) 229E and HCoV OC43 were determined by Western blotting. The Abs produced reacted with recombinant SARS virus nucleocapsid protein, but not with HCoV 229E or HCoV OC43.",

keywords = "Cross-reactivity, HCoV 229E, HCoV OC43, Recombinant nucleocapsid protein, SARS",

author = "Lee, {Hyun Kyoung} and Lee, {Byoung Hee} and Seok, {Seung Hyeok} and Baek, {Min Won} and Lee, {Hui Young} and Kim, {Dong Jae} and Na, {Yi Rang} and Noh, {Kyoung Jin} and Park, {Sung Hoon} and Kumar, {Dutta Noton} and Hiroaki Kariwa and Mina Nakauchi and Heo, {Suk Jin} and Park, {Jae Hak}",

year = "2010",

month = mar,

doi = "10.4142/jvs.2010.11.2.165",

language = "English (US)",

volume = "11",

pages = "165--167",

journal = "Journal of Veterinary Science",

issn = "1229-845X",

publisher = "Korean Society of Veterinary Science",

number = "1",

}

TY - JOUR

T1 - Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43

AU - Lee, Hyun Kyoung

AU - Lee, Byoung Hee

AU - Seok, Seung Hyeok

AU - Baek, Min Won

AU - Lee, Hui Young

AU - Kim, Dong Jae

AU - Na, Yi Rang

AU - Noh, Kyoung Jin

AU - Park, Sung Hoon

AU - Kumar, Dutta Noton

AU - Kariwa, Hiroaki

AU - Nakauchi, Mina

AU - Heo, Suk Jin

AU - Park, Jae Hak

PY - 2010/3

Y1 - 2010/3

N2 - Severe acute respiratory syndrome (SARS) is a life-threatening disease for which accurate diagnosis is essential. Although many tools have been developed for the diagnosis of SARS, false-positive reactions in negative sera may occur because of cross-reactivity with other coronaviruses. We have raised polyclonal and monoclonal antibodies (Abs) using a recombinant form of the SARS virus nucleocapsid protein. Cross-reactivity of these anti-SARS Abs against human coronavirus (HCoV) 229E and HCoV OC43 were determined by Western blotting. The Abs produced reacted with recombinant SARS virus nucleocapsid protein, but not with HCoV 229E or HCoV OC43.

AB - Severe acute respiratory syndrome (SARS) is a life-threatening disease for which accurate diagnosis is essential. Although many tools have been developed for the diagnosis of SARS, false-positive reactions in negative sera may occur because of cross-reactivity with other coronaviruses. We have raised polyclonal and monoclonal antibodies (Abs) using a recombinant form of the SARS virus nucleocapsid protein. Cross-reactivity of these anti-SARS Abs against human coronavirus (HCoV) 229E and HCoV OC43 were determined by Western blotting. The Abs produced reacted with recombinant SARS virus nucleocapsid protein, but not with HCoV 229E or HCoV OC43.

KW - Cross-reactivity

KW - HCoV 229E

KW - HCoV OC43

KW - Recombinant nucleocapsid protein

KW - SARS

UR - http://www.scopus.com/inward/record.url?scp=77952695157&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952695157&partnerID=8YFLogxK

U2 - 10.4142/jvs.2010.11.2.165

DO - 10.4142/jvs.2010.11.2.165

M3 - Article

C2 - 20458159

AN - SCOPUS:77952695157

SN - 1229-845X

VL - 11

SP - 165

EP - 167

JO - Journal of Veterinary Science

JF - Journal of Veterinary Science

IS - 1

ER -

Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this