Production of infectious hepatitis C virus in tissue culture from a cloned viral genome

Takaji Wakita, Thomas Pietschmann, Takanobu Kato, Tomoko Date, Michiko Miyamoto, Zijiang Zhao, Krishna Murthy, Anja Habermann, Hans Georg Kräusslich, Masashi Mizokami, Ralf Bartenschlager, T. Jake Liang

Research output: Contribution to journalArticle

Abstract

Hepatitis C virus (HCV) infection causes chronic liver diseases and is a global public health problem. Detailed analyses of HCV have been hampered by the lack of viral culture systems. Subgenomic replicons of the JFH1 genotype 2a strain cloned from an individual with fulminant hepatitis replicate efficiently in cell culture. Here we show that the JFH1 genome replicates efficiently and supports secretion of viral particles after transfection into a human hepatoma cell line (Huh7). Particles have a density of about 1.15-1.17 g/ml and a spherical morphology with an average diameter of about 55 nm. Secreted virus is infectious for Huh7 cells and infectivity can be neutralized by CD81-specific antibodies and by immunoglobulins from chronically infected individuals. The cell culture-generated HCV is infectious for chimpanzee. This system provides a powerful tool for studying the viral life cycle and developing antiviral strategies.

Original languageEnglish (US)
Pages (from-to)791-796
Number of pages6
JournalNature Medicine
Volume11
Issue number7
DOIs
StatePublished - Jul 2005
Externally publishedYes

Fingerprint

Hepatovirus
Tissue culture
Viral Genome
Viruses
Hepacivirus
Genes
Cell Culture Techniques
Cell culture
Replicon
Pan troglodytes
Virus Diseases
Life Cycle Stages
Virion
Hepatitis
Antiviral Agents
Transfection
Immunoglobulins
Liver Diseases
Hepatocellular Carcinoma
Public health

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Wakita, T., Pietschmann, T., Kato, T., Date, T., Miyamoto, M., Zhao, Z., ... Liang, T. J. (2005). Production of infectious hepatitis C virus in tissue culture from a cloned viral genome. Nature Medicine, 11(7), 791-796. https://doi.org/10.1038/nm1268

Production of infectious hepatitis C virus in tissue culture from a cloned viral genome. / Wakita, Takaji; Pietschmann, Thomas; Kato, Takanobu; Date, Tomoko; Miyamoto, Michiko; Zhao, Zijiang; Murthy, Krishna; Habermann, Anja; Kräusslich, Hans Georg; Mizokami, Masashi; Bartenschlager, Ralf; Liang, T. Jake.

In: Nature Medicine, Vol. 11, No. 7, 07.2005, p. 791-796.

Research output: Contribution to journalArticle

Wakita, T, Pietschmann, T, Kato, T, Date, T, Miyamoto, M, Zhao, Z, Murthy, K, Habermann, A, Kräusslich, HG, Mizokami, M, Bartenschlager, R & Liang, TJ 2005, 'Production of infectious hepatitis C virus in tissue culture from a cloned viral genome', Nature Medicine, vol. 11, no. 7, pp. 791-796. https://doi.org/10.1038/nm1268
Wakita T, Pietschmann T, Kato T, Date T, Miyamoto M, Zhao Z et al. Production of infectious hepatitis C virus in tissue culture from a cloned viral genome. Nature Medicine. 2005 Jul;11(7):791-796. https://doi.org/10.1038/nm1268
Wakita, Takaji ; Pietschmann, Thomas ; Kato, Takanobu ; Date, Tomoko ; Miyamoto, Michiko ; Zhao, Zijiang ; Murthy, Krishna ; Habermann, Anja ; Kräusslich, Hans Georg ; Mizokami, Masashi ; Bartenschlager, Ralf ; Liang, T. Jake. / Production of infectious hepatitis C virus in tissue culture from a cloned viral genome. In: Nature Medicine. 2005 ; Vol. 11, No. 7. pp. 791-796.
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