Production of IGF-II-related peptide by an anaplastic cells line (AT-3) established from the dunning prostatic carcinoma of rats

Yuhsi Matuo, Nozomu Nishi, Hideyuki Tanaka, Ikuharu Sasaki, John T. Issacs, Fumio Wada

Research output: Contribution to journalArticle

Abstract

AT-3 cells, one of anaplastic cell lines established from the Dunning prostatic carcinoma of rats, were able to grow under serum-free conditions in a state of suspension detached from a substratum. Radioimmunoassays using monoclonal antibody against rat insulin-like growth factor II (IGF-II) revealed the presence of IGF-II-related peptide in acid-ethanol extracts extracsts of lyophilized serum-free media conditioned by AT-3 cell. The peptide contents in the culture media increased with increase in cell number; 71 ng at 3.0 × 106 cells and 449 ng at 4.6 × 107 cells. IGF-II-related peptide was hardly detectable in acid-ethanol extracts of AT-3 cells harvested after 13-days culture. These results indicate that AT-3 cells produce IGF-II-related peptide ana may release it into the culture media.

Original languageEnglish (US)
Pages (from-to)1053-1056
Number of pages4
JournalIn Vitro Cellular & Developmental Biology
Volume24
Issue number10
DOIs
StatePublished - Oct 1 1988

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Keywords

  • IGF-II
  • cell culture
  • growth factors
  • prostate cancer

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

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