Production of bovine rhodopsin by mammalian cell lines expressing cloned cDNA: Spectrophotometry and subcellular localization

Jeremy Nathans, Charles J. Weitz, Neeraj Agarwal, Izhak Nir, David S. Papermaster

Research output: Contribution to journalArticlepeer-review

Abstract

Cloned cDNA encoding bovine rhodopsin has been recombined into an expression vector and cotransfected with an antibiotic resistance plasmid into cultured human embryonic kidney cells. The resulting cell lines produce 100-200 μg of bovine opsin per liter of saturated tissue culture medium (109 cells). Incubation in vitro with 11-cis retinal produces a photolabile pigment the absorbance spectrum of which is indistinguishable from that of bona fide bovine rhodopsin. Expressed rhodopsin accumulates in the plasma membrane as determined by immunoelectron microscopy.

Original languageEnglish (US)
Pages (from-to)907-914
Number of pages8
JournalVision Research
Volume29
Issue number8
DOIs
StatePublished - 1989

Keywords

  • Immunoelectron microscopy
  • Transfection
  • Visual pigment

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

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