Production of a monoclonal antibody directed against the high-affinity nerve growth factor receptor

E. Tagliabue, C. Ghirelli, L. Lombardi, F. Castiglioni, L. Asnaghi, C. Longhi, M. G. Borrello, P. Aiello, Sylvie Ménard

Research output: Contribution to journalArticlepeer-review

Abstract

The high-affinity nerve growth factor receptor corresponds to the tyrosine protein kinase encoded by the proto-oncogene trkA. Different findings suggest that nerve growth factor (NGF) can be operative in the growth modulation of tumor cell lines possessing high-affinity binding sites for this molecule. Using as immunizing material the SKNBE neuroblastoma cell line transfected with proto-trkA we produced a monoclonal antibody (MAb) able to recognize the high-affinity nerve growth factor receptor. The selected MAb, designated MGR12, is directed against an epitope present on the extracellular domain of the receptor since it showed reactivity on living trkA-expressing cells and was able to immunoprecipitate the proto-trkA molecule. The MGR12 MAb is directed against a non-functional epitope since it neither inhibited NGF binding nor induced receptor internalization. This new reagent appears to be an appropriate tool for analyzing the expression of high-affinity nerve growth factor receptor in tumors of different origin and for elucidating its involvement in tumor progression.

Original languageEnglish (US)
Pages (from-to)68-72
Number of pages5
JournalInternational Journal of Biological Markers
Volume14
Issue number2
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

Keywords

  • High-affinity nerve growth factor receptor
  • Monoclonal antibody
  • Somatic fusion

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Oncology
  • Clinical Biochemistry
  • Cancer Research

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