Production of a biologically active recombinant teleostean growth hormone in E. coli cells

Clara M. Cheng, C. M. Lin, M. Shamblott, L. I. Gonzalez-Villasenoc, D. A. Powers, C. Woods, T. T. Chen

Research output: Contribution to journalArticlepeer-review

Abstract

We have isolated and characterized several recombinant lambda phage clones carrying growth hormone (GH) cDNA of striped bass (Morone saxatilis). Nucleotide sequence and the predicted amino acid sequence of sbGH was determined from a recombinant clone carrying the longest cDNA insert. The sbGH cDNA encodes a pre-hormone of 204 amino acid residues. Comparison of the predicted amino acid sequence of sbGH with those of other vertebrates revealed different degrees of sequence identity: approximately 98% with European sea bass; 90% with bluefin tuna; bonito and red seabream; 71% with winter flounder; 64% with salmonids; 55% with carp; and 38% with human. Expression of the mature sbGH cDNA (without the signal peptide sequence) in E. coli cells under regulation of the lambda phage PL promoter produced a polypeptide of 20 kDa. Following renaturation, this recombinant hormone was shown to be biologically active in a radioreceptor competition binding assay and in the induction of hepatic insulin-like growth factor I (IGF-I) mRNA synthesis in vivo.

Original languageEnglish (US)
Pages (from-to)75-85
Number of pages11
JournalMolecular and Cellular Endocrinology
Volume108
Issue number1-2
DOIs
StatePublished - Feb 27 1995

Keywords

  • Competitive receptor binding assay
  • Expression in E. coli
  • Growth hormone
  • IGF-I induction
  • Renaturation
  • Striped bass

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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