Production and testing of an international reference standard of short ragweed pollen extract

R. M. Helm, M. B. Gauerke, H. Baer, H. Løwenstein, A. Ford, D. A. Levy, P. S. Norman, J. W. Yunginger

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


A lyophilized candidate International Reference Standard of short ragweed pollen extract was prepared by use of defined source material. In preliminary experiments, this extract was demonstrated by RAST inhibition and crossed radioimmunoelectrophoresis assays to contain several well-characterized ragweed allergens and to contain multiple antigenic bands by crossed immunoelectrophoresis analysis. In a subsequent multinational collaborative study involving 12 laboratories in five countries, the candidate extract was compared with existing national reference or commercial ragweed extracts by a variety of immunochemical, biochemical, and physicochemical procedures. The candidate extract could be used to assign relative orders of potency to the comparison-test extracts. In separate studies, the candidate extract was demonstrated to be stable when it was stored at either -20° C or +5° C for at least 2 yr. The candidate extract has been accepted as an International Reference Standard with an assigned arbitrary potency of 100,000 units per ampule.

Original languageEnglish (US)
Pages (from-to)790-800
Number of pages11
JournalThe Journal of allergy and clinical immunology
Issue number6
StatePublished - Jun 1984
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


Dive into the research topics of 'Production and testing of an international reference standard of short ragweed pollen extract'. Together they form a unique fingerprint.

Cite this