Production and significance of TGF-β in AT-3 metastatic cell line established from the Dunning rat prostatic adenocarcinoma

Yuhsi Matuo, Nozomu Nishi, Hirotoshi Takasuka, Yoshishige Masuda, Katsuzo Nishikawa, John Tod Isaacs, Pamela S. Adams, Wallace L. McKeehan, Gordon H. Sato

Research output: Contribution to journalArticle

Abstract

A colony formation assay using NRK-49F cells revealed that a metastatic cell line, AT-3, established from the Dunning prostatic carcinoma could produce TGF-β in a latent form. TGF-β at a concentration as low as 0.05 ng/ml either stimulated the attachment or detachment of AT-3 cells depending on the kind of culture media. Acid extracts from conditioned medium (5 μg/ml) showed the activity comparable to that of TGF-β (5 ng/ml). The detached cells were able to grow in suspension. TGF-β (0.1 ng/ml) could also stimulate the growth of MC3T3-El osteoblasts established from mouse calvaria. These results suggest that TGF-β is a key growth factor for osteoblastic bony metastasis of prostate cancer.

Original languageEnglish (US)
Pages (from-to)840-847
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume166
Issue number2
DOIs
StatePublished - Jan 30 1990

Fingerprint

Osteoblasts
Conditioned Culture Medium
Culture Media
Rats
Assays
Intercellular Signaling Peptides and Proteins
Suspensions
Adenocarcinoma
Cells
Cell Line
Acids
Skull
Prostatic Neoplasms
Neoplasm Metastasis
Carcinoma
Growth

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Production and significance of TGF-β in AT-3 metastatic cell line established from the Dunning rat prostatic adenocarcinoma. / Matuo, Yuhsi; Nishi, Nozomu; Takasuka, Hirotoshi; Masuda, Yoshishige; Nishikawa, Katsuzo; Isaacs, John Tod; Adams, Pamela S.; McKeehan, Wallace L.; Sato, Gordon H.

In: Biochemical and Biophysical Research Communications, Vol. 166, No. 2, 30.01.1990, p. 840-847.

Research output: Contribution to journalArticle

Matuo, Yuhsi ; Nishi, Nozomu ; Takasuka, Hirotoshi ; Masuda, Yoshishige ; Nishikawa, Katsuzo ; Isaacs, John Tod ; Adams, Pamela S. ; McKeehan, Wallace L. ; Sato, Gordon H. / Production and significance of TGF-β in AT-3 metastatic cell line established from the Dunning rat prostatic adenocarcinoma. In: Biochemical and Biophysical Research Communications. 1990 ; Vol. 166, No. 2. pp. 840-847.
@article{a5a96f617fdc49138afe687c4bf5a664,
title = "Production and significance of TGF-β in AT-3 metastatic cell line established from the Dunning rat prostatic adenocarcinoma",
abstract = "A colony formation assay using NRK-49F cells revealed that a metastatic cell line, AT-3, established from the Dunning prostatic carcinoma could produce TGF-β in a latent form. TGF-β at a concentration as low as 0.05 ng/ml either stimulated the attachment or detachment of AT-3 cells depending on the kind of culture media. Acid extracts from conditioned medium (5 μg/ml) showed the activity comparable to that of TGF-β (5 ng/ml). The detached cells were able to grow in suspension. TGF-β (0.1 ng/ml) could also stimulate the growth of MC3T3-El osteoblasts established from mouse calvaria. These results suggest that TGF-β is a key growth factor for osteoblastic bony metastasis of prostate cancer.",
author = "Yuhsi Matuo and Nozomu Nishi and Hirotoshi Takasuka and Yoshishige Masuda and Katsuzo Nishikawa and Isaacs, {John Tod} and Adams, {Pamela S.} and McKeehan, {Wallace L.} and Sato, {Gordon H.}",
year = "1990",
month = "1",
day = "30",
doi = "10.1016/0006-291X(90)90886-R",
language = "English (US)",
volume = "166",
pages = "840--847",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Production and significance of TGF-β in AT-3 metastatic cell line established from the Dunning rat prostatic adenocarcinoma

AU - Matuo, Yuhsi

AU - Nishi, Nozomu

AU - Takasuka, Hirotoshi

AU - Masuda, Yoshishige

AU - Nishikawa, Katsuzo

AU - Isaacs, John Tod

AU - Adams, Pamela S.

AU - McKeehan, Wallace L.

AU - Sato, Gordon H.

PY - 1990/1/30

Y1 - 1990/1/30

N2 - A colony formation assay using NRK-49F cells revealed that a metastatic cell line, AT-3, established from the Dunning prostatic carcinoma could produce TGF-β in a latent form. TGF-β at a concentration as low as 0.05 ng/ml either stimulated the attachment or detachment of AT-3 cells depending on the kind of culture media. Acid extracts from conditioned medium (5 μg/ml) showed the activity comparable to that of TGF-β (5 ng/ml). The detached cells were able to grow in suspension. TGF-β (0.1 ng/ml) could also stimulate the growth of MC3T3-El osteoblasts established from mouse calvaria. These results suggest that TGF-β is a key growth factor for osteoblastic bony metastasis of prostate cancer.

AB - A colony formation assay using NRK-49F cells revealed that a metastatic cell line, AT-3, established from the Dunning prostatic carcinoma could produce TGF-β in a latent form. TGF-β at a concentration as low as 0.05 ng/ml either stimulated the attachment or detachment of AT-3 cells depending on the kind of culture media. Acid extracts from conditioned medium (5 μg/ml) showed the activity comparable to that of TGF-β (5 ng/ml). The detached cells were able to grow in suspension. TGF-β (0.1 ng/ml) could also stimulate the growth of MC3T3-El osteoblasts established from mouse calvaria. These results suggest that TGF-β is a key growth factor for osteoblastic bony metastasis of prostate cancer.

UR - http://www.scopus.com/inward/record.url?scp=0025012692&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025012692&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(90)90886-R

DO - 10.1016/0006-291X(90)90886-R

M3 - Article

VL - 166

SP - 840

EP - 847

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -