Production and significance of TGF-β in AT-3 metastatic cell line established from the Dunning rat prostatic adenocarcinoma

Yuhsi Matuo, Nozomu Nishi, Hirotoshi Takasuka, Yoshishige Masuda, Katsuzo Nishikawa, John T. Isaacs, Pamela S. Adams, Wallace L. McKeehan, Gordon H. Sato

Research output: Contribution to journalArticle


A colony formation assay using NRK-49F cells revealed that a metastatic cell line, AT-3, established from the Dunning prostatic carcinoma could produce TGF-β in a latent form. TGF-β at a concentration as low as 0.05 ng/ml either stimulated the attachment or detachment of AT-3 cells depending on the kind of culture media. Acid extracts from conditioned medium (5 μg/ml) showed the activity comparable to that of TGF-β (5 ng/ml). The detached cells were able to grow in suspension. TGF-β (0.1 ng/ml) could also stimulate the growth of MC3T3-El osteoblasts established from mouse calvaria. These results suggest that TGF-β is a key growth factor for osteoblastic bony metastasis of prostate cancer.

Original languageEnglish (US)
Pages (from-to)840-847
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - Jan 30 1990


ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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