A colony formation assay using NRK-49F cells revealed that a metastatic cell line, AT-3, established from the Dunning prostatic carcinoma could produce TGF-β in a latent form. TGF-β at a concentration as low as 0.05 ng/ml either stimulated the attachment or detachment of AT-3 cells depending on the kind of culture media. Acid extracts from conditioned medium (5 μg/ml) showed the activity comparable to that of TGF-β (5 ng/ml). The detached cells were able to grow in suspension. TGF-β (0.1 ng/ml) could also stimulate the growth of MC3T3-El osteoblasts established from mouse calvaria. These results suggest that TGF-β is a key growth factor for osteoblastic bony metastasis of prostate cancer.
|Original language||English (US)|
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jan 30 1990|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology