Procoagulant Activity of Human Neuroblastoma Cell Lines, in Relation to Cell Growth, Differentiation and Cytogenetic Abnormalities

Noriko Esumi, Shinsaku Imashuku, Kentaro Tsunamoto, Shinjiro Todo, Shinichi Misawa, Toshihiro Goto, Yoshio Fujisawa

Research output: Contribution to journalArticlepeer-review

Abstract

Procoagulant activity (PCA) was investigated in relation to cell growth, differentiation, and cytogenetics in seven human neuroblastoma cell lines. Before 5‐bromodeoxyuridine (BrdUrd) treatment, PCA was notably heterogeneous, with the highest activity in NCG (S‐type in morphology) 40‐to 100‐fold greater than the lowest activity in SK‐N‐D2 (N‐type). PCA was not related to 1p abnormalities. After BrdUrd treatment at 5 μg/ml for 6 days, PCA increased 6.8‐fold in GOTO and 2.7‐fold in SK‐N‐DZ with associated growth inhibition and morphological changes (I‐type morphology converted to S‐type in GOTO and N‐type converted to an advanced N‐type in SK‐N‐DZ). In contrast, only growth suppression was observed in 2 other cell lines, and no changes in PCA, growth or morphology were induced in the remaining 3 cell lines.

Original languageEnglish (US)
Pages (from-to)438-443
Number of pages6
JournalJapanese Journal of Cancer Research
Volume80
Issue number5
DOIs
StatePublished - May 1989
Externally publishedYes

Keywords

  • 5‐Bromodeoxyuridine
  • Chromosome abnormality
  • Neuroblastoma
  • Procoagulant activity

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Procoagulant Activity of Human Neuroblastoma Cell Lines, in Relation to Cell Growth, Differentiation and Cytogenetic Abnormalities'. Together they form a unique fingerprint.

Cite this