Processing of phage T4 td-encoded RNA is analogous to the eukaryotic group I splicing pathway

Karen Ehrenman, J. Pedersen-Lane, D. West, R. Herman, F. Maley, M. Belfort

Research output: Contribution to journalArticle

Abstract

Several features of the split td gene of phage T4 suggests an RNA processing mechanism analogous to that of the self-splicing rRNA of Tetrahymena and other group I eukaryotic introns. Previous work has revealed conserved sequence elements and the ability of td-encoded RNA to self-splice in vitro. We show here that a nonencoded guanosine residue is covalently joined to the 5' end of the intron during processing. Further, we demonstrate the existence of linear and circular intron forms in RNA extracted from T4-infected cells and from uninfected Escherichia coli expressing the cloned td gene. Sequence analysis of the intron cyclization junction indicates that the noncoded guanosine and one additional nucleotide are lost from the 5' end of the intron upon cyclization. This analysis places a uridine residue upstream of the cyclization site, in analogy to three other group I cyclization junctions. These striking similarities to the splicing intermediates of eukaryotic group I introns point not only to an analogous processing pathway and conserved features of cyclization site recognition but also to a common ancestry between this prokaryotic intervening sequence and the group I eukaryotic introns.

Original languageEnglish (US)
Pages (from-to)5875-5879
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number16
DOIs
StatePublished - Dec 19 1986
Externally publishedYes

Fingerprint

Bacteriophage T4
Introns
RNA
Cyclization
Guanosine
CD4-Positive T-Lymphocytes
Tetrahymena
Conserved Sequence
Uridine
Genes
Sequence Analysis
Nucleotides
Escherichia coli

ASJC Scopus subject areas

  • General

Cite this

Processing of phage T4 td-encoded RNA is analogous to the eukaryotic group I splicing pathway. / Ehrenman, Karen; Pedersen-Lane, J.; West, D.; Herman, R.; Maley, F.; Belfort, M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 16, 19.12.1986, p. 5875-5879.

Research output: Contribution to journalArticle

@article{e12e180c56af417697e7f691a0259134,
title = "Processing of phage T4 td-encoded RNA is analogous to the eukaryotic group I splicing pathway",
abstract = "Several features of the split td gene of phage T4 suggests an RNA processing mechanism analogous to that of the self-splicing rRNA of Tetrahymena and other group I eukaryotic introns. Previous work has revealed conserved sequence elements and the ability of td-encoded RNA to self-splice in vitro. We show here that a nonencoded guanosine residue is covalently joined to the 5' end of the intron during processing. Further, we demonstrate the existence of linear and circular intron forms in RNA extracted from T4-infected cells and from uninfected Escherichia coli expressing the cloned td gene. Sequence analysis of the intron cyclization junction indicates that the noncoded guanosine and one additional nucleotide are lost from the 5' end of the intron upon cyclization. This analysis places a uridine residue upstream of the cyclization site, in analogy to three other group I cyclization junctions. These striking similarities to the splicing intermediates of eukaryotic group I introns point not only to an analogous processing pathway and conserved features of cyclization site recognition but also to a common ancestry between this prokaryotic intervening sequence and the group I eukaryotic introns.",
author = "Karen Ehrenman and J. Pedersen-Lane and D. West and R. Herman and F. Maley and M. Belfort",
year = "1986",
month = "12",
day = "19",
doi = "10.1073/pnas.83.16.5875",
language = "English (US)",
volume = "83",
pages = "5875--5879",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "16",

}

TY - JOUR

T1 - Processing of phage T4 td-encoded RNA is analogous to the eukaryotic group I splicing pathway

AU - Ehrenman, Karen

AU - Pedersen-Lane, J.

AU - West, D.

AU - Herman, R.

AU - Maley, F.

AU - Belfort, M.

PY - 1986/12/19

Y1 - 1986/12/19

N2 - Several features of the split td gene of phage T4 suggests an RNA processing mechanism analogous to that of the self-splicing rRNA of Tetrahymena and other group I eukaryotic introns. Previous work has revealed conserved sequence elements and the ability of td-encoded RNA to self-splice in vitro. We show here that a nonencoded guanosine residue is covalently joined to the 5' end of the intron during processing. Further, we demonstrate the existence of linear and circular intron forms in RNA extracted from T4-infected cells and from uninfected Escherichia coli expressing the cloned td gene. Sequence analysis of the intron cyclization junction indicates that the noncoded guanosine and one additional nucleotide are lost from the 5' end of the intron upon cyclization. This analysis places a uridine residue upstream of the cyclization site, in analogy to three other group I cyclization junctions. These striking similarities to the splicing intermediates of eukaryotic group I introns point not only to an analogous processing pathway and conserved features of cyclization site recognition but also to a common ancestry between this prokaryotic intervening sequence and the group I eukaryotic introns.

AB - Several features of the split td gene of phage T4 suggests an RNA processing mechanism analogous to that of the self-splicing rRNA of Tetrahymena and other group I eukaryotic introns. Previous work has revealed conserved sequence elements and the ability of td-encoded RNA to self-splice in vitro. We show here that a nonencoded guanosine residue is covalently joined to the 5' end of the intron during processing. Further, we demonstrate the existence of linear and circular intron forms in RNA extracted from T4-infected cells and from uninfected Escherichia coli expressing the cloned td gene. Sequence analysis of the intron cyclization junction indicates that the noncoded guanosine and one additional nucleotide are lost from the 5' end of the intron upon cyclization. This analysis places a uridine residue upstream of the cyclization site, in analogy to three other group I cyclization junctions. These striking similarities to the splicing intermediates of eukaryotic group I introns point not only to an analogous processing pathway and conserved features of cyclization site recognition but also to a common ancestry between this prokaryotic intervening sequence and the group I eukaryotic introns.

UR - http://www.scopus.com/inward/record.url?scp=0022525441&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022525441&partnerID=8YFLogxK

U2 - 10.1073/pnas.83.16.5875

DO - 10.1073/pnas.83.16.5875

M3 - Article

C2 - 3526343

AN - SCOPUS:0022525441

VL - 83

SP - 5875

EP - 5879

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 16

ER -