Processed pseudogenes acquired somatically during cancer development

Susanna L. Cooke, Adam Shlien, John Marshall, Christodoulos P. Pipinikas, Inigo Martincorena, Jose M.C. Tubio, Yilong Li, Andrew Menzies, Laura Mudie, Manasa Ramakrishna, Lucy Yates, Helen Davies, Niccolo Bolli, Graham R. Bignell, Patrick S. Tarpey, Sam Behjati, Serena Nik-Zainal, Elli Papaemmanuil, Vitor H. Teixeira, Keiran RaineSarah Oameara, Maryam S. Dodoran, Jon W. Teague, Adam P. Butler, Christine Iacobuzio-Donahue, Thomas Santarius, Richard G. Grundy, David Malkin, Mel Greaves, Nikhil Munshi, Adrienne M. Flanagan, David Bowtell, Sancha Martin, Denis Larsimont, Jorge S. Reis-Filho, Alex Boussioutas, Jack A. Taylor, Neil D. Hayes, Sam M. Janes, P. Andrew Futreal, Michael R. Stratton, Ultan McDermott, Peter J. Campbell, Elena Provenzano, Marc van de Vijver, Andrea L. Richardson, Colin Purdie, Sarah Pinder, Gaetan Mac Grogan, Anne Vincent-Salomon, Denis Larsimont, Dorthe Grabau, Torill Sauer, Øystein Garred, Anna Ehinger, Gert G. Van den Eynden, C. H.M. van Deurzen, Roberto Salgado, Jane E. Brock, Sunil R. Lakhani, Dilip D. Giri, Laurent Arnould, Jocelyne Jacquemier, Isabelle Treilleux, Carlos Caldas, Suet Feung Chin, Aquila Fatima, Alastair M. Thompson, Alasdair Stenhouse, John Foekens, John Martens, Anieta Sieuwerts, Arjen Brinkman, Henk Stunnenberg, Paul N. Span, Fred Sweep, Christine Desmedt, Christos Sotiriou, Gilles Thomas, Annegein Broeks, Anita Langerod, Samuel Aparicio, Peter T. Simpson, Laura van ’t Veer, Jorunn Erla Eyfjord, Holmfridur Hilmarsdottir, Jon G. Jonasson, Anne Lise Børresen-Dale, Ming Ta Michael Lee, Bernice Huimin Wong, Benita Kiat Tee Tan, Gerrit K.J. Hooijer

Research output: Contribution to journalArticlepeer-review

Abstract

Cancer evolves by mutation, with somatic reactivation of retrotransposons being one such mutational process. Germline retrotransposition can cause processed pseudogenes, but whether this occurs somatically has not been evaluated. Here we screen sequencing data from 660 cancer samples for somatically acquired pseudogenes. We find 42 events in 17 samples, especially non-small cell lung cancer (5/27) and colorectal cancer (2/11). Genomic features mirror those of germline LINE element retrotranspositions, with frequent target-site duplications (67%), consensus TTTTAA sites at insertion points, inverted rearrangements (21%), 5a €2 truncation (74%) and polyA tails (88%). Transcriptional consequences include expression of pseudogenes from UTRs or introns of target genes. In addition, a somatic pseudogene that integrated into the promoter and first exon of the tumour suppressor gene, MGA, abrogated expression from that allele. Thus, formation of processed pseudogenes represents a new class of mutation occurring during cancer development, with potentially diverse functional consequences depending on genomic context.

Original languageEnglish (US)
Article number3644
JournalNature communications
Volume5
DOIs
StatePublished - Apr 9 2014

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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