Processed allografts and type I collagen conduits for repair of peripheral nerve gaps

Elizabeth L. Whitlock, Sami Tuffaha, Janina P. Luciano, Ying Yan, Daniel A. Hunter, Christina K. Magill, Amy M. Moore, Alice Y. Tong, Susan E. Mackinnon, Gregory H. Borschel

Research output: Contribution to journalArticle

Abstract

Autografting is the gold standard in the repair of peripheral nerve injuries that are not amenable to end-to-end coaptation. However, because autografts result in donor-site defects and are a limited resource, an effective substitute would be valuable. In a rat model, we compared isografts with Integra NeuraGen® (NG) nerve guides, which are a commercially available type I collagen conduit, with processed rat allografts comparable to AxoGen's Avance® human decellularized allograft product. In a 14-mm sciatic nerve gap model, isograft was superior to processed allograft, which was in turn superior to NG conduit at 6 weeks postoperatively (P < 0.05 for number of myelinated fibers both at midgraft and distal to the graft). At 12 weeks, these differences were no longer apparent. In a 28-mm graft model, isografts again performed better than processed allografts at both 6 and 22 weeks; regeneration through the NG conduit was often insufficient for analysis in this long graft model. Functional tests confirmed the superiority of isografts, although processed allografts permitted successful reinnervation of distal targets not seen in the NG conduit groups. Processed allografts were inherently non-immunogenic and maintained some internal laminin structure. We conclude that, particularly in a long gap model, nerve graft alternatives fail to confer the regenerative advantages of an isograft. However, AxoGen processed allografts are superior to a currently available conduit-style nerve guide, the Integra NeuraGen®. They provide an alternative for reconstruction of short nerve gaps where a conduit might otherwise be used.

Original languageEnglish (US)
Pages (from-to)787-799
Number of pages13
JournalMuscle and Nerve
Volume39
Issue number6
DOIs
StatePublished - Jun 1 2009
Externally publishedYes

Fingerprint

Collagen Type I
Isografts
Peripheral Nerves
Allografts
Transplants
Peripheral Nerve Injuries
Autologous Transplantation
Autografts
Laminin
Sciatic Nerve
Regeneration

Keywords

  • Avance®
  • Nerve allograft
  • Nerve bank
  • Nerve tissue engineering
  • NeuraGen®

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)

Cite this

Whitlock, E. L., Tuffaha, S., Luciano, J. P., Yan, Y., Hunter, D. A., Magill, C. K., ... Borschel, G. H. (2009). Processed allografts and type I collagen conduits for repair of peripheral nerve gaps. Muscle and Nerve, 39(6), 787-799. https://doi.org/10.1002/mus.21220

Processed allografts and type I collagen conduits for repair of peripheral nerve gaps. / Whitlock, Elizabeth L.; Tuffaha, Sami; Luciano, Janina P.; Yan, Ying; Hunter, Daniel A.; Magill, Christina K.; Moore, Amy M.; Tong, Alice Y.; Mackinnon, Susan E.; Borschel, Gregory H.

In: Muscle and Nerve, Vol. 39, No. 6, 01.06.2009, p. 787-799.

Research output: Contribution to journalArticle

Whitlock, EL, Tuffaha, S, Luciano, JP, Yan, Y, Hunter, DA, Magill, CK, Moore, AM, Tong, AY, Mackinnon, SE & Borschel, GH 2009, 'Processed allografts and type I collagen conduits for repair of peripheral nerve gaps', Muscle and Nerve, vol. 39, no. 6, pp. 787-799. https://doi.org/10.1002/mus.21220
Whitlock, Elizabeth L. ; Tuffaha, Sami ; Luciano, Janina P. ; Yan, Ying ; Hunter, Daniel A. ; Magill, Christina K. ; Moore, Amy M. ; Tong, Alice Y. ; Mackinnon, Susan E. ; Borschel, Gregory H. / Processed allografts and type I collagen conduits for repair of peripheral nerve gaps. In: Muscle and Nerve. 2009 ; Vol. 39, No. 6. pp. 787-799.
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