Probing vesicle dynamics in single hippocampal synapses

Matthew Shtrahman, Chuck Yeung, David Nauen, Guo Qiang Bi, Xiao Lun Wu

Research output: Contribution to journalArticle

Abstract

We use fluorescence correlation spectroscopy and fluorescence recovery after photobleaching to study vesicle dynamics inside the synapses of cultured hippocampal neurons labeled with the fluorescent vesicle marker FM 1-43. These studies show that when the cell is electrically at rest, only a small population of vesicles is mobile, taking seconds to traverse the synapse. Applying the phosphatase inhibitor okadaic acid causes vesicles to diffuse freely, moving 30 times faster than vesicles in control synapses. These results suggest that vesicles move sluggishly due to binding to elements of the synaptic cytomatrix and that this binding is altered by phosphorylation. Motivated by these results, a model is constructed consisting of diffusing vesicles that bind reversibly to the cytomatrix. This stick-and-diffuse model accounts for the fluorescence correlation spectroscopy and fluorescence recovery after photobleaching data, and also predicts the well-known exponential refilling of the readily releasable pool. Our measurements suggest that the movement of vesicles to the active zone is the rate-limiting step in this process.

Original languageEnglish (US)
Pages (from-to)3615-3627
Number of pages13
JournalBiophysical Journal
Volume89
Issue number5
DOIs
StatePublished - Nov 2005
Externally publishedYes

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Synapses
Fluorescence Recovery After Photobleaching
Fluorescence Spectrometry
Okadaic Acid
Phosphoric Monoester Hydrolases
Phosphorylation
Neurons
Population
FM1 43

ASJC Scopus subject areas

  • Biophysics

Cite this

Probing vesicle dynamics in single hippocampal synapses. / Shtrahman, Matthew; Yeung, Chuck; Nauen, David; Bi, Guo Qiang; Wu, Xiao Lun.

In: Biophysical Journal, Vol. 89, No. 5, 11.2005, p. 3615-3627.

Research output: Contribution to journalArticle

Shtrahman, Matthew ; Yeung, Chuck ; Nauen, David ; Bi, Guo Qiang ; Wu, Xiao Lun. / Probing vesicle dynamics in single hippocampal synapses. In: Biophysical Journal. 2005 ; Vol. 89, No. 5. pp. 3615-3627.
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