Probing the structure of RecA-DNA filaments. Advantages of a fluorescent guanine analog

Scott F. Singleton, Alberto I. Roca, Andrew M. Lee, Jie Xiao

Research output: Contribution to journalArticle

Abstract

The RecA protein of Escherichia coli plays a crucial role in DNA recombination and repair, as well as various aspects of bacterial pathogenicity. The formation of a RecA-ATP-ssDNA complex initiates all RecA activities and yet a complete structural and mechanistic description of this filament has remained elusive. An analysis of RecA-DNA interactions was performed using fluorescently labeled oligonucleotides. A direct comparison was made between fluorescein and several fluorescent nucleosides. The fluorescent guanine analog 6-methylisoxanthopterin (6MI) demonstrated significant advantages over the other fluorophores and represents an important new tool for characterizing RecA-DNA interactions.

Original languageEnglish (US)
Pages (from-to)3553-3566
Number of pages14
JournalTetrahedron
Volume63
Issue number17
DOIs
Publication statusPublished - Apr 23 2007

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Keywords

  • DNA repair
  • Fluorescence
  • Pteridine nucleoside
  • Recombination

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Drug Discovery

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