Probing the structure of RecA-DNA filaments. Advantages of a fluorescent guanine analog

Scott F. Singleton, Alberto I. Roca, Andrew M. Lee, Jie Xiao

Research output: Contribution to journalArticle

Abstract

The RecA protein of Escherichia coli plays a crucial role in DNA recombination and repair, as well as various aspects of bacterial pathogenicity. The formation of a RecA-ATP-ssDNA complex initiates all RecA activities and yet a complete structural and mechanistic description of this filament has remained elusive. An analysis of RecA-DNA interactions was performed using fluorescently labeled oligonucleotides. A direct comparison was made between fluorescein and several fluorescent nucleosides. The fluorescent guanine analog 6-methylisoxanthopterin (6MI) demonstrated significant advantages over the other fluorophores and represents an important new tool for characterizing RecA-DNA interactions.

Original languageEnglish (US)
Pages (from-to)3553-3566
Number of pages14
JournalTetrahedron
Volume63
Issue number17
DOIs
StatePublished - Apr 23 2007

Fingerprint

Guanine
Rec A Recombinases
Recombinational DNA Repair
DNA
Fluorescein
Nucleosides
Oligonucleotides
DNA Repair
Virulence
Fluorophores
Adenosine Triphosphate
Escherichia coli
Repair
6-methylisoxanthopterin

Keywords

  • DNA repair
  • Fluorescence
  • Pteridine nucleoside
  • Recombination

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Drug Discovery

Cite this

Probing the structure of RecA-DNA filaments. Advantages of a fluorescent guanine analog. / Singleton, Scott F.; Roca, Alberto I.; Lee, Andrew M.; Xiao, Jie.

In: Tetrahedron, Vol. 63, No. 17, 23.04.2007, p. 3553-3566.

Research output: Contribution to journalArticle

Singleton, Scott F. ; Roca, Alberto I. ; Lee, Andrew M. ; Xiao, Jie. / Probing the structure of RecA-DNA filaments. Advantages of a fluorescent guanine analog. In: Tetrahedron. 2007 ; Vol. 63, No. 17. pp. 3553-3566.
@article{5b76df7d9f4c45639e37b1e2696c3d97,
title = "Probing the structure of RecA-DNA filaments. Advantages of a fluorescent guanine analog",
abstract = "The RecA protein of Escherichia coli plays a crucial role in DNA recombination and repair, as well as various aspects of bacterial pathogenicity. The formation of a RecA-ATP-ssDNA complex initiates all RecA activities and yet a complete structural and mechanistic description of this filament has remained elusive. An analysis of RecA-DNA interactions was performed using fluorescently labeled oligonucleotides. A direct comparison was made between fluorescein and several fluorescent nucleosides. The fluorescent guanine analog 6-methylisoxanthopterin (6MI) demonstrated significant advantages over the other fluorophores and represents an important new tool for characterizing RecA-DNA interactions.",
keywords = "DNA repair, Fluorescence, Pteridine nucleoside, Recombination",
author = "Singleton, {Scott F.} and Roca, {Alberto I.} and Lee, {Andrew M.} and Jie Xiao",
year = "2007",
month = "4",
day = "23",
doi = "10.1016/j.tet.2006.10.092",
language = "English (US)",
volume = "63",
pages = "3553--3566",
journal = "Tetrahedron",
issn = "0040-4020",
publisher = "Elsevier Limited",
number = "17",

}

TY - JOUR

T1 - Probing the structure of RecA-DNA filaments. Advantages of a fluorescent guanine analog

AU - Singleton, Scott F.

AU - Roca, Alberto I.

AU - Lee, Andrew M.

AU - Xiao, Jie

PY - 2007/4/23

Y1 - 2007/4/23

N2 - The RecA protein of Escherichia coli plays a crucial role in DNA recombination and repair, as well as various aspects of bacterial pathogenicity. The formation of a RecA-ATP-ssDNA complex initiates all RecA activities and yet a complete structural and mechanistic description of this filament has remained elusive. An analysis of RecA-DNA interactions was performed using fluorescently labeled oligonucleotides. A direct comparison was made between fluorescein and several fluorescent nucleosides. The fluorescent guanine analog 6-methylisoxanthopterin (6MI) demonstrated significant advantages over the other fluorophores and represents an important new tool for characterizing RecA-DNA interactions.

AB - The RecA protein of Escherichia coli plays a crucial role in DNA recombination and repair, as well as various aspects of bacterial pathogenicity. The formation of a RecA-ATP-ssDNA complex initiates all RecA activities and yet a complete structural and mechanistic description of this filament has remained elusive. An analysis of RecA-DNA interactions was performed using fluorescently labeled oligonucleotides. A direct comparison was made between fluorescein and several fluorescent nucleosides. The fluorescent guanine analog 6-methylisoxanthopterin (6MI) demonstrated significant advantages over the other fluorophores and represents an important new tool for characterizing RecA-DNA interactions.

KW - DNA repair

KW - Fluorescence

KW - Pteridine nucleoside

KW - Recombination

UR - http://www.scopus.com/inward/record.url?scp=33947192465&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33947192465&partnerID=8YFLogxK

U2 - 10.1016/j.tet.2006.10.092

DO - 10.1016/j.tet.2006.10.092

M3 - Article

C2 - 17955055

AN - SCOPUS:33947192465

VL - 63

SP - 3553

EP - 3566

JO - Tetrahedron

JF - Tetrahedron

SN - 0040-4020

IS - 17

ER -