Probing the interaction between single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor

S. Weiss; T. Ha; Th Enderle; D. F. Ogletree; P. R. Selvin; D. S. Chemla

Probing the interaction between single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor

S. Weiss, T. Ha, Th Enderle, D. F. Ogletree, P. R. Selvin, D. S. Chemla

Research output: Contribution to conference › Paper › peer-review

Abstract

Fluorescence resonance energy transfer is a standard spectroscopic technique for measuring distances in the 10-75 angstrom range. This study extends the technique's sensitivity by measuring energy transfer between a single donor fluorophore and a single acceptor fluorophore. Near-field scanning optical microscopy was used to obtain simultaneous dual color imaging and emission spectra from a single pair of donor and acceptor fluorophores linked by a short (10 or 20 bases long) DNA molecule. The DNA was dried on amino-propyl-silanized glass. Near-field fluorescence images and emission spectra were taken on single DNA molecules labeled with donor only, with acceptor only, or with both donor and acceptor.

Original language	English (US)
Number of pages	1
State	Published - Jan 1 1996
Externally published	Yes
Event	Proceedings of the 1996 6th Quantum Electronics and Laser Science Conference, QELS - Anaheim, CA, USA Duration: Jun 2 1996 → Jun 7 1996

Conference

Conference	Proceedings of the 1996 6th Quantum Electronics and Laser Science Conference, QELS
City	Anaheim, CA, USA
Period	6/2/96 → 6/7/96

ASJC Scopus subject areas

General Physics and Astronomy

Cite this

Probing the interaction between single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor. / Weiss, S.; Ha, T.; Enderle, Th et al.
1996. Paper presented at Proceedings of the 1996 6th Quantum Electronics and Laser Science Conference, QELS, Anaheim, CA, USA.

Research output: Contribution to conference › Paper › peer-review

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abstract = "Fluorescence resonance energy transfer is a standard spectroscopic technique for measuring distances in the 10-75 angstrom range. This study extends the technique's sensitivity by measuring energy transfer between a single donor fluorophore and a single acceptor fluorophore. Near-field scanning optical microscopy was used to obtain simultaneous dual color imaging and emission spectra from a single pair of donor and acceptor fluorophores linked by a short (10 or 20 bases long) DNA molecule. The DNA was dried on amino-propyl-silanized glass. Near-field fluorescence images and emission spectra were taken on single DNA molecules labeled with donor only, with acceptor only, or with both donor and acceptor.",

author = "S. Weiss and T. Ha and Th Enderle and Ogletree, {D. F.} and Selvin, {P. R.} and Chemla, {D. S.}",

year = "1996",

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T2 - Proceedings of the 1996 6th Quantum Electronics and Laser Science Conference, QELS

AU - Weiss, S.

AU - Ha, T.

AU - Enderle, Th

AU - Ogletree, D. F.

AU - Selvin, P. R.

AU - Chemla, D. S.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Fluorescence resonance energy transfer is a standard spectroscopic technique for measuring distances in the 10-75 angstrom range. This study extends the technique's sensitivity by measuring energy transfer between a single donor fluorophore and a single acceptor fluorophore. Near-field scanning optical microscopy was used to obtain simultaneous dual color imaging and emission spectra from a single pair of donor and acceptor fluorophores linked by a short (10 or 20 bases long) DNA molecule. The DNA was dried on amino-propyl-silanized glass. Near-field fluorescence images and emission spectra were taken on single DNA molecules labeled with donor only, with acceptor only, or with both donor and acceptor.

AB - Fluorescence resonance energy transfer is a standard spectroscopic technique for measuring distances in the 10-75 angstrom range. This study extends the technique's sensitivity by measuring energy transfer between a single donor fluorophore and a single acceptor fluorophore. Near-field scanning optical microscopy was used to obtain simultaneous dual color imaging and emission spectra from a single pair of donor and acceptor fluorophores linked by a short (10 or 20 bases long) DNA molecule. The DNA was dried on amino-propyl-silanized glass. Near-field fluorescence images and emission spectra were taken on single DNA molecules labeled with donor only, with acceptor only, or with both donor and acceptor.

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M3 - Paper

AN - SCOPUS:0029695890

Y2 - 2 June 1996 through 7 June 1996

ER -

Probing the interaction between single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor

Abstract

Conference

ASJC Scopus subject areas

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