Probing T cell membrane organization using dimeric MHC-Ig complexes

Tarek M. Fahmy, Joan G. Bieler, Jonathan P. Schneck

Research output: Contribution to journalArticle

Abstract

In this report, we review a novel method for probing the membrane organization of T cells using dimeric major histocompatibility complexes (MHC), MHC-Ig. MHC-Ig complexes are useful reagents for quantitative analysis of binding data since their valency is controlled. These complexes can be easily labeled and loaded with a variety of peptides. A binding assay using these dimers and quantitative analysis of the MHC-Ig dimer-T cell binding curves is described in detail. Using this approach, we show that the organization of TCR on activated T cells is different from TCR organization on naïve T cells. The implications of these findings are discussed with regards to current models of T cell recognition. This analysis offers insight into how T cell controls their biological range of responsiveness. Specifically, these findings reveal the biophysical basis of the ability of activated T cells to recognize low amounts of antigen independent of costimulation.

Original languageEnglish (US)
Pages (from-to)93-106
Number of pages14
JournalJournal of Immunological Methods
Volume268
Issue number1
DOIs
StatePublished - Oct 1 2002

Keywords

  • Binding assay
  • Clusters
  • Crosslinks
  • Dimeric MHC
  • Fusion constructs
  • Lipid rafts
  • MHC-Ig
  • Sensitivity
  • T cells
  • TCR organization

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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