Probing single helicase dynamics on long nucleic acids through fluorescence-force measurement

Chang Ting Lin, Taekjip Ha

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Helicases are nucleic acid-dependent ATPases which can bind and remodel nucleic acids, protein–nucleic acid complexes, or both. They are involved in almost every step in cells related to nucleic acid metabolisms, including DNA replication and repair, transcription, RNA maturation and splicing, and nuclear export processes. Using single-molecule fluorescence-force spectroscopy, we have previously directly observed helicase translocation on long single-stranded DNA and revealed that two monomers of UvrD helicase are required for the initiation of unwinding function. Here, we present the details of fluorescence-force spectroscopy instrumentation, calibration, and activity assays in detail for observing the biochemical activities of helicases in real time and revealing how mechanical forces are involved in protein–nucleic acid interaction. These single-molecule approaches are generally applicable to many other protein–nucleic acid systems.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages295-316
Number of pages22
Volume1486
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1486
ISSN (Print)10643745

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Keywords

  • Fluorescence localization
  • Helicase
  • NS3
  • Optical tweezers
  • Single-molecule
  • TIRF
  • Translocation
  • Unwinding
  • UvrD

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Lin, C. T., & Ha, T. (2017). Probing single helicase dynamics on long nucleic acids through fluorescence-force measurement. In Methods in Molecular Biology (Vol. 1486, pp. 295-316). (Methods in Molecular Biology; Vol. 1486). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6421-5_11