Probing phosphorylation by non-mammalian isoprenoid biosynthetic enzymes using 1H-31P-31P correlation NMR spectroscopy

Ananya Majumdar, Meha H. Shah, J. Kipchirchir Bitok, Maria E. Hassis-Lebeau, Caren L Meyers

Research output: Contribution to journalArticle

Abstract

The biogenesis of isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) is accomplished by the methylerythritol phosphate (MEP) pathway in plants, bacteria and parasites, making it a potential target for the development of anti-infective agents and herbicides. The biosynthetic enzymes comprising this pathway catalyze intriguing chemical transformations on diphosphate scaffolds, offering an opportunity to generate novel analogs in this synthetically challenging compound class. Such a biosynthetic approach to generating new diphosphate analogs may involve transformation through discrete diphosphate species, presenting unique challenges in structure determination and characterization of unnatural enzyme-generated diphosphate products produced in tandem. We have developed 1H-31P-31P correlation NMR spectroscopy techniques for the direct characterization of crude MEP pathway enzyme products at low concentrations (200 μM to 5 mM) on a room temperature (non-cryogenic) NMR probe. Coupling the 100% natural abundance of the 31P nucleus with the high intrinsic sensitivity of proton NMR, 1H-31P-31P correlation spectroscopy is particularly useful for characterization of unnatural diphosphate enzyme products in the MEP pathway. As proof of principle, we demonstrate the rapid characterization of natural enzyme products of the enzymes IspD, E and F in tandem enzyme incubations. In addition, we have characterized several unnatural enzyme products using this technique, including new products of cytidyltransferase IspD bearing erythritol, glycerol and ribose components. The results of this study indicate that IspD may be a useful biocatalyst and highlight 1H-31P-31P correlation spectroscopy as a valuable tool for the characterization of other unnatural products in non-mammalian isoprenoid biosynthesis.

Original languageEnglish (US)
Pages (from-to)935-944
Number of pages10
JournalMolecular BioSystems
Volume5
Issue number9
DOIs
StatePublished - 2009

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Terpenes
Magnetic Resonance Spectroscopy
Phosphorylation
Diphosphates
Enzymes
Phosphates
Spectrum Analysis
Erythritol
Ribose
Herbicides
Anti-Infective Agents
Glycerol
Protons
Parasites
Bacteria
Temperature

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

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Probing phosphorylation by non-mammalian isoprenoid biosynthetic enzymes using 1H-31P-31P correlation NMR spectroscopy. / Majumdar, Ananya; Shah, Meha H.; Bitok, J. Kipchirchir; Hassis-Lebeau, Maria E.; Meyers, Caren L.

In: Molecular BioSystems, Vol. 5, No. 9, 2009, p. 935-944.

Research output: Contribution to journalArticle

Majumdar, Ananya ; Shah, Meha H. ; Bitok, J. Kipchirchir ; Hassis-Lebeau, Maria E. ; Meyers, Caren L. / Probing phosphorylation by non-mammalian isoprenoid biosynthetic enzymes using 1H-31P-31P correlation NMR spectroscopy. In: Molecular BioSystems. 2009 ; Vol. 5, No. 9. pp. 935-944.
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