Probing Intercellular Interactions between Vascular Endothelial Cadherin Pairs at Single-molecule Resolution and in Living Cells

Porntula Panorchan, Jerry P. George, Denis Wirtz

Research output: Contribution to journalArticle

Abstract

Vascular endothelial (VE) cadherin is the surface glycoprotein cadherin specific to the endothelium that mediates cell-cell adhesion and plays a major role in the remodeling, gating, and maturation of vascular vessels. To investigate the contribution of individual VE-cadherins to endothelial cell-cell interactions and investigate whether different classical cadherins display different kinetics and micromechanical properties, we characterize the binding properties of VE-cadherin/VE-cadherin bonds at single-molecule resolution and in living human umbilical vein endothelial cells (HUVECs). Our single-molecule force spectroscopy measurements reveal that type II VE-cadherin molecules form bonds that are less prone to rupture and display a higher tensile strength than bonds formed by classical type I neuronal (N) cadherin and epithelial (E) cadherin. The equilibrium lifetime of the VE-cadherin/VE-cadherin bond is significantly longer than formed by N-cadherin/N-cadherin bonds and E-cadherin/E-cadherin bonds. These results indicate that VE-cadherins form bonds that have kinetics and mechanical properties that are significantly different from those formed by classical type I cadherins, properties that are particularly well adapted to the barrier and adhesive functions of VE-cadherin in endothelial cell-cell junctions.

Original languageEnglish (US)
Pages (from-to)665-674
Number of pages10
JournalJournal of Molecular Biology
Volume358
Issue number3
DOIs
StatePublished - May 5 2006

Fingerprint

Cadherins
Endothelial Cells
cadherin 5
Intercellular Junctions
Tensile Strength
Membrane Glycoproteins
Human Umbilical Vein Endothelial Cells
Cell Adhesion
Cell Communication
Adhesives
Endothelium
Blood Vessels
Rupture

Keywords

  • cell adhesion
  • endothelium
  • single-molecule spectroscopy
  • VE-cadherin

ASJC Scopus subject areas

  • Virology

Cite this

Probing Intercellular Interactions between Vascular Endothelial Cadherin Pairs at Single-molecule Resolution and in Living Cells. / Panorchan, Porntula; George, Jerry P.; Wirtz, Denis.

In: Journal of Molecular Biology, Vol. 358, No. 3, 05.05.2006, p. 665-674.

Research output: Contribution to journalArticle

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