Probing domain swapping for the neuronal SNARE complex with electron paramagnetic resonance

Dae Hyuk Kweon, Yong Chen, Fan Zhang, Michelle Poirier, Chang Sup Kim, Yeon Kyun Shin

Research output: Contribution to journalArticlepeer-review

Abstract

Highly conserved soluble N-ethylmaleimide sensitive factor attachment protein receptor (SNARE) proteins control membrane fusion at synapses. The target plasma membrane-associated SNARE proteins and the vesicle-associated SNARE protein assemble into a parallel four-helix bundle. Using a novel EPR approach, it is found that the SNARE four-helix bundles are interconnected via domain swapping that is achieved by substituting one of the two SNAP-25 helices with the identical helix from the second four-helical bundle. Domain swapping is likely to play a role in the multimerization of the SNARE complex that is required for successful membrane fusion. The new EPR application employed here should be useful to study other polymerizing proteins.

Original languageEnglish (US)
Pages (from-to)5449-5452
Number of pages4
JournalBiochemistry
Volume41
Issue number17
DOIs
StatePublished - Apr 30 2002

ASJC Scopus subject areas

  • Biochemistry

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