Pro-B-cell-specific transcription and proapoptotic function of protein kinase Cη

Theresa A. Morrow, Stefan A. Muljo, Jun Zhang, J Marie Hardwick, Mark S. Schlissel

Research output: Contribution to journalArticle

Abstract

Using a subtractive cloning scheme on cDNA prepared from primary pro-B and pre-B cells, we identified several genes whose products regulate apoptosis. We further characterized one of these genes, encoding protein kinase Cη (PKCη). PKCη transcripts were readily detected in pro-B cells but were absent in pre-B cells. Although both a full-length and a truncated form of PKCη were detectable in bone marrow pro-B cells, transition to the pre-B-cell stage was associated with increased relative levels of truncated PKCη. We found that PKCη is proteolyzed in apoptotic lymphocytes, generating a kinase-active fragment identical to the truncated form which is capable of inducing apoptosis when expressed in a pro-B cell line. Caspase-3 can generate an identical PKCη cleavage product in vitro, and caspase inhibitors prevent the generation of this product during apoptosis in transfected cell lines. Inducible overexpression of either the full-length or truncated form of PKCη results in cell cycle arrest at the G1/S transition. These results suggest that the expression and proteolytic activation of PKCη play an important role in the regulation of cell division and cell death during early B-cell development.

Original languageEnglish (US)
Pages (from-to)5608-5618
Number of pages11
JournalMolecular and Cellular Biology
Volume19
Issue number8
StatePublished - Aug 1999

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B-Lymphoid Precursor Cells
Protein Kinase C
Apoptosis
G1 Phase Cell Cycle Checkpoints
Cell Line
Caspase Inhibitors
Caspase 3
Bone Marrow Cells
Cell Division
Organism Cloning
B-Lymphocytes
Cell Death
Phosphotransferases
Complementary DNA
Lymphocytes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Morrow, T. A., Muljo, S. A., Zhang, J., Hardwick, J. M., & Schlissel, M. S. (1999). Pro-B-cell-specific transcription and proapoptotic function of protein kinase Cη. Molecular and Cellular Biology, 19(8), 5608-5618.

Pro-B-cell-specific transcription and proapoptotic function of protein kinase Cη. / Morrow, Theresa A.; Muljo, Stefan A.; Zhang, Jun; Hardwick, J Marie; Schlissel, Mark S.

In: Molecular and Cellular Biology, Vol. 19, No. 8, 08.1999, p. 5608-5618.

Research output: Contribution to journalArticle

Morrow, TA, Muljo, SA, Zhang, J, Hardwick, JM & Schlissel, MS 1999, 'Pro-B-cell-specific transcription and proapoptotic function of protein kinase Cη', Molecular and Cellular Biology, vol. 19, no. 8, pp. 5608-5618.
Morrow, Theresa A. ; Muljo, Stefan A. ; Zhang, Jun ; Hardwick, J Marie ; Schlissel, Mark S. / Pro-B-cell-specific transcription and proapoptotic function of protein kinase Cη. In: Molecular and Cellular Biology. 1999 ; Vol. 19, No. 8. pp. 5608-5618.
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