Pro-apoptotic action of Par-4 involves inhibition of NF-κB activity and suppression of Bcl-2 expression

Simonetta Camandola, Mark P. Mattson

Research output: Contribution to journalArticle

Abstract

Par-41 (prostate apoptosis response 4) is known to function at an early stage in apoptosis in several different cell types, including neurons. On the other hand, activation of the transcription factor NF-κB can prevent apoptosis in various cancer cells and neurons. We now report that overexpression of full-length Par-4 in cultured PC12 cells results in a suppression of basal NF-κB DNA-binding activity and NF-κB activation following trophic factor withdrawal (TFW). The decreased NF-κB activity is correlated with enhanced apoptosis. Conversely, NF-κB activity is increased and vulnerability to apoptosis reduced in cells overexpressing a dominant- negative form of Par-4. Par-4 overexpression or functional blockade had no effect on AP-1 DNA-binding activity. Expression of the antiapoptotic protein Bcl-2 was dramatically reduced in PC12 cells overexpressing Par-4. Our data suggest that suppression of NF-κB activation plays a major role in the proapoptotic function of Par-4. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)134-139
Number of pages6
JournalJournal of Neuroscience Research
Volume61
Issue number2
DOIs
StatePublished - Jul 15 2000
Externally publishedYes

Fingerprint

Apoptosis
PC12 Cells
Neurons
DNA
Transcription Factor AP-1
Prostate
Cultured Cells
Transcription Factors
Neoplasms
Proteins

Keywords

  • Apoptosis
  • Kinase
  • Mitochondria
  • Neurons
  • Neurotrophic factors

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Pro-apoptotic action of Par-4 involves inhibition of NF-κB activity and suppression of Bcl-2 expression. / Camandola, Simonetta; Mattson, Mark P.

In: Journal of Neuroscience Research, Vol. 61, No. 2, 15.07.2000, p. 134-139.

Research output: Contribution to journalArticle

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N2 - Par-41 (prostate apoptosis response 4) is known to function at an early stage in apoptosis in several different cell types, including neurons. On the other hand, activation of the transcription factor NF-κB can prevent apoptosis in various cancer cells and neurons. We now report that overexpression of full-length Par-4 in cultured PC12 cells results in a suppression of basal NF-κB DNA-binding activity and NF-κB activation following trophic factor withdrawal (TFW). The decreased NF-κB activity is correlated with enhanced apoptosis. Conversely, NF-κB activity is increased and vulnerability to apoptosis reduced in cells overexpressing a dominant- negative form of Par-4. Par-4 overexpression or functional blockade had no effect on AP-1 DNA-binding activity. Expression of the antiapoptotic protein Bcl-2 was dramatically reduced in PC12 cells overexpressing Par-4. Our data suggest that suppression of NF-κB activation plays a major role in the proapoptotic function of Par-4. (C) 2000 Wiley-Liss, Inc.

AB - Par-41 (prostate apoptosis response 4) is known to function at an early stage in apoptosis in several different cell types, including neurons. On the other hand, activation of the transcription factor NF-κB can prevent apoptosis in various cancer cells and neurons. We now report that overexpression of full-length Par-4 in cultured PC12 cells results in a suppression of basal NF-κB DNA-binding activity and NF-κB activation following trophic factor withdrawal (TFW). The decreased NF-κB activity is correlated with enhanced apoptosis. Conversely, NF-κB activity is increased and vulnerability to apoptosis reduced in cells overexpressing a dominant- negative form of Par-4. Par-4 overexpression or functional blockade had no effect on AP-1 DNA-binding activity. Expression of the antiapoptotic protein Bcl-2 was dramatically reduced in PC12 cells overexpressing Par-4. Our data suggest that suppression of NF-κB activation plays a major role in the proapoptotic function of Par-4. (C) 2000 Wiley-Liss, Inc.

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