PRKX critically regulates endothelial cell proliferation, migration, and vascular-like structure formation

Xiaohong Li, Carlo Iomini, Deborah Hyink, Patricia D. Wilson

Research output: Contribution to journalArticle

Abstract

Angiogenesis is a fundamental step in several important physiological events and pathological conditions including embryonic development, wound repair, tumor growth and metastasis. PRKX was identified as a novel type-I cAMP-dependent protein kinase gene expressed in multiple developing tissues. PRKX has also been shown to be phylogenetically and functionally distinct from PKA. This study presents the first evidence that PRKX stimulates endothelial cell proliferation, migration, and vascular-like structure formation, which are the three essential processes for angiogenesis. In contrast, classic PKA demonstrated an inhibitory effect on endothelia vascular-like structure formation. Our findings suggest that PRKX is an important protein kinase engaged in the regulation of angiogenesis and could play critical roles in various physiological and pathological conditions involving angiogenesis. PRKX binds to Pin-1, Magi-1 and Bag-3, which regulate cell proliferation, apoptosis, differentiation and tumorigenesis. The interaction of PRKX with Pin-1, Magi-1 and Bag-3 could contribute to the stimulating role of PRKX in angiogenesis.

Original languageEnglish (US)
Pages (from-to)475-485
Number of pages11
JournalDevelopmental biology
Volume356
Issue number2
DOIs
Publication statusPublished - Jan 1 2011
Externally publishedYes

    Fingerprint

Keywords

  • Angiogenesis
  • Cell adhesion
  • Endothelial cell migration
  • PRKX
  • Proliferation

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this