Primate cytomegalovirus assembly: Evidence that DNA packaging occurs subsequent to B capsid assembly

Joanna Y. Lee, Alice Irmiere, Wade Gibson

Research output: Contribution to journalArticle

Abstract

Results presented here show that when cytomegalovirus (strain Colburn)-infected cells are treated with the DNA synthesis inhibitor hydroxyurea or phosphonoformate, one type of intranuclear capsid accumulates. These particles appeared to contain symmetrically organized internal material, and had a protein composition and sedimentation rate characteristic of B capsids. Radiolabeling experiments provided evidence that a population of B capsids lacking DNA is present during the course of a normal infection. These capsids sedimented slightly slower than the peak of viral DNA in the same region of the gradient, and had a ratio of DNA/protein that was estimated to be sevenfold lower than that of the faster sedimenting C capsids. DNA in both the B and C capsid regions of such gradients was found to be relatively resistant to digestion with DNase. The possibility is considered that herpesvirus B capsids lacking DNA may be counterparts of unexpanded proheads in the bacteriophage assembly pathway.

Original languageEnglish (US)
Pages (from-to)87-96
Number of pages10
JournalVirology
Volume167
Issue number1
DOIs
StatePublished - Nov 1988

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ASJC Scopus subject areas

  • Virology

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