Primate cytomegalovirus assembly: Evidence that DNA packaging occurs subsequent to B capsid assembly

Joanna Y. Lee, Alice Irmiere, D Wade Gibson

Research output: Contribution to journalArticle

Abstract

Results presented here show that when cytomegalovirus (strain Colburn)-infected cells are treated with the DNA synthesis inhibitor hydroxyurea or phosphonoformate, one type of intranuclear capsid accumulates. These particles appeared to contain symmetrically organized internal material, and had a protein composition and sedimentation rate characteristic of B capsids. Radiolabeling experiments provided evidence that a population of B capsids lacking DNA is present during the course of a normal infection. These capsids sedimented slightly slower than the peak of viral DNA in the same region of the gradient, and had a ratio of DNA/protein that was estimated to be sevenfold lower than that of the faster sedimenting C capsids. DNA in both the B and C capsid regions of such gradients was found to be relatively resistant to digestion with DNase. The possibility is considered that herpesvirus B capsids lacking DNA may be counterparts of unexpanded proheads in the bacteriophage assembly pathway.

Original languageEnglish (US)
Pages (from-to)87-96
Number of pages10
JournalVirology
Volume167
Issue number1
DOIs
StatePublished - 1988

Fingerprint

DNA Packaging
Capsid
Cytomegalovirus
Primates
DNA
Foscarnet
Nucleic Acid Synthesis Inhibitors
Cercopithecine Herpesvirus 1
Hydroxyurea
Deoxyribonucleases
Viral DNA
Bacteriophages
Digestion
Proteins
Infection

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Primate cytomegalovirus assembly : Evidence that DNA packaging occurs subsequent to B capsid assembly. / Lee, Joanna Y.; Irmiere, Alice; Gibson, D Wade.

In: Virology, Vol. 167, No. 1, 1988, p. 87-96.

Research output: Contribution to journalArticle

@article{5a136551b8b748dbae251188fb98e272,
title = "Primate cytomegalovirus assembly: Evidence that DNA packaging occurs subsequent to B capsid assembly",
abstract = "Results presented here show that when cytomegalovirus (strain Colburn)-infected cells are treated with the DNA synthesis inhibitor hydroxyurea or phosphonoformate, one type of intranuclear capsid accumulates. These particles appeared to contain symmetrically organized internal material, and had a protein composition and sedimentation rate characteristic of B capsids. Radiolabeling experiments provided evidence that a population of B capsids lacking DNA is present during the course of a normal infection. These capsids sedimented slightly slower than the peak of viral DNA in the same region of the gradient, and had a ratio of DNA/protein that was estimated to be sevenfold lower than that of the faster sedimenting C capsids. DNA in both the B and C capsid regions of such gradients was found to be relatively resistant to digestion with DNase. The possibility is considered that herpesvirus B capsids lacking DNA may be counterparts of unexpanded proheads in the bacteriophage assembly pathway.",
author = "Lee, {Joanna Y.} and Alice Irmiere and Gibson, {D Wade}",
year = "1988",
doi = "10.1016/0042-6822(88)90057-8",
language = "English (US)",
volume = "167",
pages = "87--96",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Primate cytomegalovirus assembly

T2 - Evidence that DNA packaging occurs subsequent to B capsid assembly

AU - Lee, Joanna Y.

AU - Irmiere, Alice

AU - Gibson, D Wade

PY - 1988

Y1 - 1988

N2 - Results presented here show that when cytomegalovirus (strain Colburn)-infected cells are treated with the DNA synthesis inhibitor hydroxyurea or phosphonoformate, one type of intranuclear capsid accumulates. These particles appeared to contain symmetrically organized internal material, and had a protein composition and sedimentation rate characteristic of B capsids. Radiolabeling experiments provided evidence that a population of B capsids lacking DNA is present during the course of a normal infection. These capsids sedimented slightly slower than the peak of viral DNA in the same region of the gradient, and had a ratio of DNA/protein that was estimated to be sevenfold lower than that of the faster sedimenting C capsids. DNA in both the B and C capsid regions of such gradients was found to be relatively resistant to digestion with DNase. The possibility is considered that herpesvirus B capsids lacking DNA may be counterparts of unexpanded proheads in the bacteriophage assembly pathway.

AB - Results presented here show that when cytomegalovirus (strain Colburn)-infected cells are treated with the DNA synthesis inhibitor hydroxyurea or phosphonoformate, one type of intranuclear capsid accumulates. These particles appeared to contain symmetrically organized internal material, and had a protein composition and sedimentation rate characteristic of B capsids. Radiolabeling experiments provided evidence that a population of B capsids lacking DNA is present during the course of a normal infection. These capsids sedimented slightly slower than the peak of viral DNA in the same region of the gradient, and had a ratio of DNA/protein that was estimated to be sevenfold lower than that of the faster sedimenting C capsids. DNA in both the B and C capsid regions of such gradients was found to be relatively resistant to digestion with DNase. The possibility is considered that herpesvirus B capsids lacking DNA may be counterparts of unexpanded proheads in the bacteriophage assembly pathway.

UR - http://www.scopus.com/inward/record.url?scp=0023811818&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023811818&partnerID=8YFLogxK

U2 - 10.1016/0042-6822(88)90057-8

DO - 10.1016/0042-6822(88)90057-8

M3 - Article

C2 - 2847426

AN - SCOPUS:0023811818

VL - 167

SP - 87

EP - 96

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1

ER -