Primary structure and functional expression of a cyclic nucleotide-activated channel from olfactory neurons

Ravinder S. Dhallan, King-Wai Yau, Karen A. Schrader, Randall R Reed

Research output: Contribution to journalArticle

Abstract

ODORANT signal transduction occurs in the specialized cilia of the olfactory sensory neurons. Considerable biochemical evidence now indicates that this process could be mediated by a G protein-coupled cascade using cyclic AMP as an intracellular second messenger1. A stimulatory G protein α subunit is expressed at high levels in olfactory neurons and is specifically enriched in the cilia2, as is a novel form of adenylyl cyclase3. This implies that the olfactory transduction cascade might involve unique molecular components. Electrophysiological studies have identified a cyclic nucleotide-activated ion channel in olfactory cilia4. These observations provide evidence for a model in which odorants increase intracellular cAMP concentration, which in turn activates this channel and depolarizes the sensory neuron. An analogous cascade regulating a cGMP-gated channel mediates visual transduction in photoreceptor cells (see refs 5,6 for review). The formal similarities between olfactory and visual transduction suggest that the two systems might use homologous channels. Here we report the molecular cloning, functional expression and characterization of a channel that is likely to mediate olfactory transduction.

Original languageEnglish (US)
Pages (from-to)184-187
Number of pages4
JournalNature
Volume347
Issue number6289
StatePublished - Sep 13 1990

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Cyclic Nucleotides
GTP-Binding Proteins
Olfactory Receptor Neurons
Neurons
Photoreceptor Cells
Cilia
Protein Subunits
Molecular Cloning
Sensory Receptor Cells
Ion Channels
Cyclic AMP
Signal Transduction
Odorants

ASJC Scopus subject areas

  • General

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Primary structure and functional expression of a cyclic nucleotide-activated channel from olfactory neurons. / Dhallan, Ravinder S.; Yau, King-Wai; Schrader, Karen A.; Reed, Randall R.

In: Nature, Vol. 347, No. 6289, 13.09.1990, p. 184-187.

Research output: Contribution to journalArticle

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