Primary structure and functional expression of a cortical collecting duct K(ir) channel

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Abstract

Maintenance of a negative membrane potential in the cortical collecting duct (CCD) principal cell depends on a small-conductance, inward-rectifying basolateral membrane K+ (K(ir)) channel. In the present study, a candidate cDNA encoding this K+ channel, CCD-IRK3, was isolated from a mouse collecting duct cell line, M1. CCD-IRK3 shares a high degree of homology with a human brain inward-rectifier K+ channel (K(ir) 2.3). By Northern analysis, CCD-IRK3 transcript (2.9 kb) was readily detected in M1 CCD cells but not in Madin-Darby canine kidney, LLC-PK1, Chinese hamster ovary, or monkey kidney fibroblast cell lines. CCD-IRK3-specific reverse transcription-polymerase chain reaction confirmed bonafide expression in the kidney. Functional expression studies in Xenopus oocytes revealed that CCD- IRK3 operates as strongly inward-rectifying K+ channel. The cation selectivity profile of CCD-IRK3[ionic permeability values (P(K)/P(i)), Tl ≤ Rb ≤ K+ >> NH4 > Na; inward-slope conductance (G(K)/G(i)), Tl ≤ K+ >> NH4 > Na > Rb] is similiar to the macroscopic CCD basolateral membrane K+ conductance (G(K)/G(i), K+ >> NH4 > Rb; P(K)/P(i), Rb ≃ K+ >> NH4). CCD- IRK3 also exhibits the pharmacological features of the native channel. Patch-damp analysis reveals that CCD-IRK3 functions as a high open probability, voltage-independent, small-conductance channel (14.5 pS), consistent with the native channel. Based on these independent lines of evidence, CCD-IRK3 is a possible candidate for the small-conductance basolateral K(ir) channel in the CCD.

Original languageEnglish (US)
Pages (from-to)F825-F836
JournalAmerican Journal of Physiology - Renal Physiology
Volume273
Issue number5 42-5
DOIs
StatePublished - 1997
Externally publishedYes

Keywords

  • Epithelial transport
  • Fluid and electrolyte balance
  • Inward-rectifying potassium channel
  • Kidney

ASJC Scopus subject areas

  • Physiology
  • Urology

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