Primary structure and functional expression of a cGMP-gated potassium channel

Xiaoqiang Yao, Alan S. Segal, Paul Welling, Xiaoqin Zhang, Carmel M. Mcnicholas, David Engel, Emile L. Boulpaep, Gary V. Desir

Research output: Contribution to journalArticle

Abstract

Cyclic nucleotides modulate potassium (K) channel activity in many cells and are thought to act indirectly by inducing channel protein phosphorylation. Herein we report the isolation from rabbit of a gene encoding a K channel (Kcn1) that is specifically activated by cGMP and not by cAMP. Analysis of the deduced amino acid sequence (725 amino acids) indicates that, in addition to a core region that is highly homologous to Shaker K channels, Kcn1 also contains a cysteine-rich region similar to that of ligand-gated ion channels and a cyclic nucleotide-binding region. Northern blot analysis detects gene expression in kidney, aorta, and brain. Kcn1 represents a class of K channels that may be specifically regulated by cGMP and could play an important role in mediating the effects of substances, such as nitric oxide, that increase intracellular cGMP.

Original languageEnglish (US)
Pages (from-to)11711-11715
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number25
DOIs
StatePublished - Dec 5 1995
Externally publishedYes

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ASJC Scopus subject areas

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