Primary culture of neonatal rat olfactory neurons

G. V. Ronnett, L. D. Hester, S. H. Snyder

Research output: Contribution to journalArticlepeer-review

Abstract

We have prepared primary cultures of purified neonatal rat olfactory neurons. Dissociated olfactory epithelial cells are maintained in modified Eagle's medium with D-valine, cytosine arabinoside, and NGF. NGF is required for neuronal survival. Immunohistochemical staining is positive for the neuronal markers vimentin, olfactory marker protein, and neuron-specific enolase, but negative for the glial markers, glial fibrillary acidic protein, and S-100 protein. Physiologic concentrations of odorants stimulate cAMP accumulation in the cells. Because of their morphology, biochemical composition, and responsiveness to odorants, these cells should enhance olfactory investigations.

Original languageEnglish (US)
Pages (from-to)1243-1255
Number of pages13
JournalJournal of Neuroscience
Volume11
Issue number5
DOIs
StatePublished - 1991

ASJC Scopus subject areas

  • Neuroscience(all)

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