Primary culture of capillary endothelium from rat brain

Phillip D. Bowman, A. Lorris Betz, Diane aR, Jerry S. Wolinsky, Jack B. Penney, Richard R. Shivers, Gary W. Goldstein

Research output: Contribution to journalArticlepeer-review

Abstract

To provide an in vitro system for studies of brain capillary function we developed a method for culture of brain capillary endothelial cells. Capillaries were isolated from rat brain and enzymatically treated to remove the basement membrane and contaminating pericytes. Subsequent Percoll gradient centrifugation resulted in a homogeneous population of capillary endothelial cells that attached to a collagen substrate and incorporated [3H]thymidine. Evidence for the endothelial nature of these cells was provided by the presence of Factor VIII antigen and angiotensin converting enzyme activity and by the failure of platelets to adhere to the cell surface. In addition, the cells were joined together by tight junctions. Thus, primary cultures of these cells retained both endothelial and blood-brain barrier features.

Original languageEnglish (US)
Pages (from-to)353-362
Number of pages10
JournalIn Vitro
Volume17
Issue number4
DOIs
StatePublished - Apr 1981
Externally publishedYes

Keywords

  • Factor VIII
  • angiotensin converting enzyme
  • endothelial cell culture
  • primary culture
  • rat brain
  • tight junctions

ASJC Scopus subject areas

  • Biotechnology
  • Plant Science

Fingerprint

Dive into the research topics of 'Primary culture of capillary endothelium from rat brain'. Together they form a unique fingerprint.

Cite this