Primary cilia of human endothelial cells disassemble under laminar shear stress

Carlo Iomini, Karla Tejada, Wenjun Mo, Heikki Vaananen, Gianni Piperno

Research output: Contribution to journalArticle

Abstract

We identified primary cilia and centrosomes in cultured human umbilical vein endothelial cells (HUVEC) by antibodies to acetyl-α-tubulin and capillary morphogenesis gene-1 product (CMG-1), a human homologue of the intraflagellar transport (IFT) protein IFT-71 in Chlamydomonas. CMG-1 was present in particles along primary cilia of HUVEC at interphase and around the oldest basal body/centriole at interphase and mitosis. To study the response of primary cilia and centrosomes to mechanical stimuli, we exposed cultured HUVEC to laminar shear stress (LSS). Under LSS, all primary cilia disassembled, and centrosomes were deprived of CMG-1. We conclude that the exposure to LSS ends the IFT in cultured endothelial cells.

Original languageEnglish (US)
Pages (from-to)811-817
Number of pages7
JournalJournal of Cell Biology
Volume164
Issue number6
DOIs
StatePublished - Mar 15 2004
Externally publishedYes

Fingerprint

Cilia
Centrosome
Human Umbilical Vein Endothelial Cells
Endothelial Cells
Morphogenesis
Interphase
Genes
Basal Bodies
Centrioles
Chlamydomonas
Protein Transport
Tubulin
Mitosis
Cultured Cells
Carrier Proteins
Antibodies

Keywords

  • Chlamydomonas
  • CMG-1
  • HUVEC
  • IFT
  • PKD-1

ASJC Scopus subject areas

  • Cell Biology

Cite this

Primary cilia of human endothelial cells disassemble under laminar shear stress. / Iomini, Carlo; Tejada, Karla; Mo, Wenjun; Vaananen, Heikki; Piperno, Gianni.

In: Journal of Cell Biology, Vol. 164, No. 6, 15.03.2004, p. 811-817.

Research output: Contribution to journalArticle

Iomini, Carlo ; Tejada, Karla ; Mo, Wenjun ; Vaananen, Heikki ; Piperno, Gianni. / Primary cilia of human endothelial cells disassemble under laminar shear stress. In: Journal of Cell Biology. 2004 ; Vol. 164, No. 6. pp. 811-817.
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