TY - JOUR
T1 - Prevention or moderation of some ultrastructural changes in the RPE and retina of galactosemic rats by aldose reductase inhibition
AU - Vinores, Stanley A.
AU - Campochiaro, Peter A.
N1 - Funding Information:
This study was supported in part by PHS Diabetes and Research Training Grant’ AM 22125, by PHS grant EY-05951 (P.A.C.). by a feasibility grant from the American Diabetes Association (P.A.C.) and an unrestricted grant from Research to Prevent Blindness, Inc. Preliminary results of this study were presented at the 1987 meeting of the Association for Research in Vision and Ophthalmology (ARVO).
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1989/9
Y1 - 1989/9
N2 - Rats were maintained on a 50% galactose diet with and without the aldose reductase inhibitor, Sorbinil, or on a normal diet of rat chow, and the retinas and retinal pigment epithelium were examined ultrastructurally at time points ranging from 4 weeks to 20 months. Several ultrastructural changes were observed in the retinal pigment epithelium and retinal capillaries of galactosemic rats that were not seen in rats on a normal diet. Only some of these changes are similar to those that have been previously noted in diabetic (glucosemic) rats. As in diabetics, galactosemic rats demonstrated thickening of the basal laminae of the retinal pigment epithelium and retinal capillaries. They also manifested vacuolization and degenerative foci in the retinal pigment epithelium that were similar, but not identical, to changes seen in diabetic rats. Each of these changes was significantly inhibited by Sorbinil. Unlike diabetics, galactosemic rats did not have dilated basal infoldings, but did have outer retinal folds and a significant increase in large lipofuscin-like aggregates in the retinal pigment epithelium, both of which were partly prevented by Sorbinil. These data suggest that multiple mechanisms may be involved in retinal and retinal pigment epithelium changes in diabetic and galactosemic rats, and that enhanced polyol metabolism is likely to be involved in some of the changes in both.
AB - Rats were maintained on a 50% galactose diet with and without the aldose reductase inhibitor, Sorbinil, or on a normal diet of rat chow, and the retinas and retinal pigment epithelium were examined ultrastructurally at time points ranging from 4 weeks to 20 months. Several ultrastructural changes were observed in the retinal pigment epithelium and retinal capillaries of galactosemic rats that were not seen in rats on a normal diet. Only some of these changes are similar to those that have been previously noted in diabetic (glucosemic) rats. As in diabetics, galactosemic rats demonstrated thickening of the basal laminae of the retinal pigment epithelium and retinal capillaries. They also manifested vacuolization and degenerative foci in the retinal pigment epithelium that were similar, but not identical, to changes seen in diabetic rats. Each of these changes was significantly inhibited by Sorbinil. Unlike diabetics, galactosemic rats did not have dilated basal infoldings, but did have outer retinal folds and a significant increase in large lipofuscin-like aggregates in the retinal pigment epithelium, both of which were partly prevented by Sorbinil. These data suggest that multiple mechanisms may be involved in retinal and retinal pigment epithelium changes in diabetic and galactosemic rats, and that enhanced polyol metabolism is likely to be involved in some of the changes in both.
KW - basement membranes
KW - diabetic retinopathy
KW - galactosemia
KW - retina
KW - retinal pigment epithelium
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U2 - 10.1016/0014-4835(89)90057-2
DO - 10.1016/0014-4835(89)90057-2
M3 - Article
C2 - 2507341
AN - SCOPUS:0024443920
SN - 0014-4835
VL - 49
SP - 495
EP - 510
JO - Experimental eye research
JF - Experimental eye research
IS - 3
ER -