Preventing the loss of competence for neural induction: HGF/SF, L5 and Sox-2

Andrea Streit, Shanthini Sockanathan, Lidia Pérez, Maria Rex, Paul J. Scotting, Paul T. Sharpe, Robin Lovell-Badge, Claudio D. Stern

Research output: Contribution to journalArticle

Abstract

The response to neural induction depends on the presence of inducing signals and on the state of competence of the responding tissue. The epiblast of the chick embryo loses its ability to respond to neural induction by the organizer (Hensen's node) between stages 4 and 4+. We find that the pattern of expression of the L5220 antigen closely mirrors the changes in competence of the epiblast in time and in space. For the first time, we describe an experiment that can extend the period of neural competence: when L5220 expression is maintained beyond its normal time by implanting HGF/SF secreting cells, the competence to respond to Hensen's node grafts is retained. The host epiblast forms a non-regionalized neural tube, which expresses the pan-neural marker SOX-2 (a Sry-related transcription factor) but not any region-specific markers for the forebrain, hindbrain or spinal cord. Although HGF/SF secreting cells can mimic signals from Hensen's node that maintain L5 expression, they cannot rescue the ability of the node to induce anterior structures (which is normally lost after stage 4). The ectoderm may acquire stable neural characteristics during neural induction by going through a hierarchy of states: competence, neuralization and regionalization. Our findings allow us to start to define these different states at a molecular level, and show that the competence to respond to neural induction is not entirely autonomous to the responding cells, but can be regulated by extracellular signalling molecules.

Original languageEnglish (US)
Pages (from-to)1191-1202
Number of pages12
JournalDevelopment
Volume124
Issue number6
StatePublished - Mar 1997
Externally publishedYes

Fingerprint

Mental Competency
Embryonic Organizers
Germ Layers
Aptitude
Rhombencephalon
Ectoderm
Neural Tube
Chick Embryo
Prosencephalon
Spinal Cord
Transcription Factors
Transplants
Antigens

Keywords

  • Chick embryo
  • Competence
  • Hensen's node
  • HGF/SF
  • Neural induction
  • Sox-2

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Streit, A., Sockanathan, S., Pérez, L., Rex, M., Scotting, P. J., Sharpe, P. T., ... Stern, C. D. (1997). Preventing the loss of competence for neural induction: HGF/SF, L5 and Sox-2. Development, 124(6), 1191-1202.

Preventing the loss of competence for neural induction : HGF/SF, L5 and Sox-2. / Streit, Andrea; Sockanathan, Shanthini; Pérez, Lidia; Rex, Maria; Scotting, Paul J.; Sharpe, Paul T.; Lovell-Badge, Robin; Stern, Claudio D.

In: Development, Vol. 124, No. 6, 03.1997, p. 1191-1202.

Research output: Contribution to journalArticle

Streit, A, Sockanathan, S, Pérez, L, Rex, M, Scotting, PJ, Sharpe, PT, Lovell-Badge, R & Stern, CD 1997, 'Preventing the loss of competence for neural induction: HGF/SF, L5 and Sox-2', Development, vol. 124, no. 6, pp. 1191-1202.
Streit A, Sockanathan S, Pérez L, Rex M, Scotting PJ, Sharpe PT et al. Preventing the loss of competence for neural induction: HGF/SF, L5 and Sox-2. Development. 1997 Mar;124(6):1191-1202.
Streit, Andrea ; Sockanathan, Shanthini ; Pérez, Lidia ; Rex, Maria ; Scotting, Paul J. ; Sharpe, Paul T. ; Lovell-Badge, Robin ; Stern, Claudio D. / Preventing the loss of competence for neural induction : HGF/SF, L5 and Sox-2. In: Development. 1997 ; Vol. 124, No. 6. pp. 1191-1202.
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