Prevalence of norovirus among visitors from the United States to Mexico and Guatemala who experience traveler's diarrhea

Amy R. Chapin; Colleen M. Carpenter; William C. Dudley; Lucy C. Gibson; Rafael Pratdesaba; Olga Torres; Domingo Sanchez; Jaime Belkind-Gerson; Irene Nyquist; Anders Kärnell; Bjorn Gustafsson; Jane L. Halpern; A. Louis Bourgeois; Kellogg J. Schwab

doi:10.1128/JCM.43.3.1112-1117.2005

Prevalence of norovirus among visitors from the United States to Mexico and Guatemala who experience traveler's diarrhea

Amy R. Chapin, Colleen M. Carpenter, William C. Dudley, Lucy C. Gibson, Rafael Pratdesaba, Olga Torres, Domingo Sanchez, Jaime Belkind-Gerson, Irene Nyquist, Anders Kärnell, Bjorn Gustafsson, Jane L. Halpern, A. Louis Bourgeois, Kellogg J. Schwab

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

Traveler's diarrhea (TD) is the most common infectious illness acquired by visitors to developing nations. The purpose of this study was to utilize molecular diagnostic techniques to determine the prevalence of norovirus (NoV) in TD occurring among visitors from the United States to Guatemala and Mexico. Stool samples (n = 54) were collected from 34 TD cases and analyzed for NoV by reverse transcription-PCR and oligoprobe confirmation. The overall prevalence of NoV was 65%. Interestingly, all NoV-positive stool samples were identified as genogroup I NoVs, and time spent at travel destinations was found to be an important factor in determining the frequency of infection (P = 0.003). Eleven NoV-positive stool samples also tested positive for enterotoxigenic Escherichia coli, indicating that dual infections with this leading bacterial cause of TD were very common. Results of this study suggest that NoV infection is a frequent occurrence among travelers to Mexico and Guatemala who experience episodes of TD. In addition, the simple molecular detection method utilized here will serve to facilitate more in-depth epidemiological studies of this emergent viral pathogen in travelers and other at-risk populations.

Original language	English (US)
Pages (from-to)	1112-1117
Number of pages	6
Journal	Journal of clinical microbiology
Volume	43
Issue number	3
DOIs	https://doi.org/10.1128/JCM.43.3.1112-1117.2005
State	Published - Mar 2005

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/JCM.43.3.1112-1117.2005

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Chapin, A. R., Carpenter, C. M., Dudley, W. C., Gibson, L. C., Pratdesaba, R., Torres, O., Sanchez, D., Belkind-Gerson, J., Nyquist, I., Kärnell, A., Gustafsson, B., Halpern, J. L., Bourgeois, A. L., & Schwab, K. J. (2005). Prevalence of norovirus among visitors from the United States to Mexico and Guatemala who experience traveler's diarrhea. Journal of clinical microbiology, 43(3), 1112-1117. https://doi.org/10.1128/JCM.43.3.1112-1117.2005

Chapin, AR, Carpenter, CM, Dudley, WC, Gibson, LC, Pratdesaba, R, Torres, O, Sanchez, D, Belkind-Gerson, J, Nyquist, I, Kärnell, A, Gustafsson, B, Halpern, JL, Bourgeois, AL & Schwab, KJ 2005, 'Prevalence of norovirus among visitors from the United States to Mexico and Guatemala who experience traveler's diarrhea', Journal of clinical microbiology, vol. 43, no. 3, pp. 1112-1117. https://doi.org/10.1128/JCM.43.3.1112-1117.2005

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abstract = "Traveler's diarrhea (TD) is the most common infectious illness acquired by visitors to developing nations. The purpose of this study was to utilize molecular diagnostic techniques to determine the prevalence of norovirus (NoV) in TD occurring among visitors from the United States to Guatemala and Mexico. Stool samples (n = 54) were collected from 34 TD cases and analyzed for NoV by reverse transcription-PCR and oligoprobe confirmation. The overall prevalence of NoV was 65%. Interestingly, all NoV-positive stool samples were identified as genogroup I NoVs, and time spent at travel destinations was found to be an important factor in determining the frequency of infection (P = 0.003). Eleven NoV-positive stool samples also tested positive for enterotoxigenic Escherichia coli, indicating that dual infections with this leading bacterial cause of TD were very common. Results of this study suggest that NoV infection is a frequent occurrence among travelers to Mexico and Guatemala who experience episodes of TD. In addition, the simple molecular detection method utilized here will serve to facilitate more in-depth epidemiological studies of this emergent viral pathogen in travelers and other at-risk populations.",

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AU - Bourgeois, A. Louis

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Prevalence of norovirus among visitors from the United States to Mexico and Guatemala who experience traveler's diarrhea

Abstract

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