Prevalence and distribution of 15 high-risk human papillomavirus types in squamous cell carcinoma of the scrotum

Andres Matoso, Maria Valeria Fabre, M. Ruhul Quddus, Marcos Lepe, Kara A. Lombardo, Pradip Manna, Jonathan Ira Epstein

Research output: Contribution to journalArticle


Which subtype(s) of high-risk human papillomavirus (hrHPV) are involved in squamous cell carcinoma (SCC) of the scrotum is unknown. Twenty-seven cases of SCC of the scrotum were retrieved, and all 15 subtypes of hrHPV and their viral loads were assessed using multiplex real-time polymerase chain reaction. The results were correlated with the histopathologic features, p16 expression, and in situ hybridization for hrHPV. hrHPV was identified in 18 (67%) of 27 of the cases, including HPV16 (n = 8), HPV35 (n = 7), HPV31 (n = 5), HPV59 (n = 5), HPV33 (n = 3), HPV18 (n = 2), HPV51 (n = 2), HPV39 (n = 1), HPV56 (n = 1), and HPV82 (n = 1). Of the 18 cases, 10 (56%) were infected by multiple hrHPV subtypes. In situ carcinomas had higher viral loads than invasive (50 M versus 2 M in average). The average age of HPV-positive and -negative cases was similar, 55 and 51, respectively. Of 11 cases of invasive carcinoma, 5 (45%) were positive for hrHPV versus 13 of 16 (81%) of in situ carcinomas. The highest proportion of hrHPV-positive cases was seen in basaloid type (7/7; 100%) and warty type (4/4; 100%), followed by usual type (7/16; 44%). Of 18 of the HPV-positive cases, 9 (50%) were also positive for p16 by immunohistochemistry and 6 of 18 (33%) were positive by in situ hybridization. Similar to SCC of the vulva and penis, the most frequently HPV-positive tumors are basaloid and warty types. However, a proportion of SCC usual type are also positive for hrHPV. Our results show that 8 (44%) of 18 of cases are associated with hrHPV subtypes other than 16 and 18. Additionally, 7 (70%) of 10 of hrHPV16/18-positive cases are coinfected with other subtypes.

Original languageEnglish (US)
Pages (from-to)130-136
Number of pages7
JournalHuman Pathology
StatePublished - Jul 1 2016


  • HPV
  • p16
  • Scrotum
  • Squamous cell carcinoma
  • Vaccine

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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