Presentation of lipoteichoic acid potentiates its inflammatory activity

Susanne Deininger, Stephanie Traub, Diana Aichele, Tamara Rupp, Teodora Baris, Heiko M. Möller, Thomas Hartung, Sonja von Aulock

Research output: Contribution to journalArticle

Abstract

Lipoteichoic acid (LTA) is a major immunostimulatory molecule in the cell wall of Gram-positive bacteria. Adhesion of LTA to a polystyrene surface drastically increased its immunostimulatory potency in human whole blood in comparison to soluble LTA, although only 1% of the LTA had bound, as determined using rhodamine-labelled LTA. The release of the proinflammatory cytokines IL-1β, TNF and IL-6 and the chemokines IL-8 and G-CSF was increased 2- to 10-fold, but IL-10 release was unaltered. This presentation effect was not shared by lipopolysaccharide (LPS) or other toll-like receptor 2 agonists and was less pronounced in polypropylene vessels. LTA did not induce cytokine release in silicone-coated borosilicate vessels, but covalent coupling of LTA to polystyrene beads restored cytokine induction in these vessels, indicating that presentation of LTA on a surface is in fact essential for its immunostimulatory potency. This novel aspect of presentation as a factor in the recognition of LTA may reflect the physiological situation in the bacterial cell wall, where LTA is anchored in the bacterial membrane and projects through the peptidoglycan. In practical terms, contamination of medical devices with components of Gram-positive bacteria may pose an underestimated inflammatory risk.

Original languageEnglish (US)
Pages (from-to)519-529
Number of pages11
JournalImmunobiology
Volume213
Issue number6
DOIs
StatePublished - Jul 10 2008
Externally publishedYes

Fingerprint

Polystyrenes
Gram-Positive Bacteria
Cytokines
Cell Wall
lipoteichoic acid
Toll-Like Receptor 2
Rhodamines
Peptidoglycan
Polypropylenes
Granulocyte Colony-Stimulating Factor
Silicones
Interleukin-8
Interleukin-1
Chemokines
Interleukin-10
Lipopolysaccharides
Interleukin-6
Equipment and Supplies
Membranes

Keywords

  • Bacteria
  • Cytokines
  • Inflammation
  • Lipoteichoic acid
  • Potency

ASJC Scopus subject areas

  • Immunology

Cite this

Deininger, S., Traub, S., Aichele, D., Rupp, T., Baris, T., Möller, H. M., ... von Aulock, S. (2008). Presentation of lipoteichoic acid potentiates its inflammatory activity. Immunobiology, 213(6), 519-529. https://doi.org/10.1016/j.imbio.2008.01.001

Presentation of lipoteichoic acid potentiates its inflammatory activity. / Deininger, Susanne; Traub, Stephanie; Aichele, Diana; Rupp, Tamara; Baris, Teodora; Möller, Heiko M.; Hartung, Thomas; von Aulock, Sonja.

In: Immunobiology, Vol. 213, No. 6, 10.07.2008, p. 519-529.

Research output: Contribution to journalArticle

Deininger, S, Traub, S, Aichele, D, Rupp, T, Baris, T, Möller, HM, Hartung, T & von Aulock, S 2008, 'Presentation of lipoteichoic acid potentiates its inflammatory activity', Immunobiology, vol. 213, no. 6, pp. 519-529. https://doi.org/10.1016/j.imbio.2008.01.001
Deininger S, Traub S, Aichele D, Rupp T, Baris T, Möller HM et al. Presentation of lipoteichoic acid potentiates its inflammatory activity. Immunobiology. 2008 Jul 10;213(6):519-529. https://doi.org/10.1016/j.imbio.2008.01.001
Deininger, Susanne ; Traub, Stephanie ; Aichele, Diana ; Rupp, Tamara ; Baris, Teodora ; Möller, Heiko M. ; Hartung, Thomas ; von Aulock, Sonja. / Presentation of lipoteichoic acid potentiates its inflammatory activity. In: Immunobiology. 2008 ; Vol. 213, No. 6. pp. 519-529.
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