Presence of the 55 kDa glycosylation inhibiting factor in human serum

Kaoru Kasahara; Tatsumi Nakano; Hiroki Takahashi; Yasuyuki Ishii; Kimishige Ishizaka; Kohzoh Imai

doi:10.1093/intimm/12.9.1303

Presence of the 55 kDa glycosylation inhibiting factor in human serum

Kaoru Kasahara, Tatsumi Nakano, Hiroki Takahashi, Yasuyuki Ishii, Kimishige Ishizaka, Kohzoh Imai

Research output: Contribution to journal › Article › peer-review

Abstract

An ELISA system for the human glycosylation inhibiting factor (GIF) was established using polyclonal antibodies against highly purified 13 kDa recombinant human GIF, and the concentration of GIF in the sera of healthy donors and patients with various diseases was determined. GIF was detected in the sera of most healthy individuals and its concentration tended to increase with age. It was also found that the serum GIF levels markedly increased in some patients with rheumatoid arthritis or malignant tumors. Analysis of serum samples by SDS-PAGE and immunoblotting revealed a 55 kDa protein that has both the GIF antigenic determinant and the TCR α chain determinant. A 13 kDa GIF was not detected in the sera. In view of our previous findings on antigen-specific GIF from murine suppressor T cell hybridomas indicating that the 55 kDa GIF is a post-translationaly formed conjugate of a TCR α chain with 13 kDa GIF, we suspect that the 55 kDa GIF detected in human sera is a human homologue of the murine 55 kDa GIF.

Original language	English (US)
Pages (from-to)	1303-1309
Number of pages	7
Journal	International Immunology
Volume	12
Issue number	9
DOIs	https://doi.org/10.1093/intimm/12.9.1303
State	Published - 2000
Externally published	Yes

Keywords

Aging
Malignant tumor
Rheumatoid arthritis
T(S) cell

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1093/intimm/12.9.1303

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title = "Presence of the 55 kDa glycosylation inhibiting factor in human serum",

abstract = "An ELISA system for the human glycosylation inhibiting factor (GIF) was established using polyclonal antibodies against highly purified 13 kDa recombinant human GIF, and the concentration of GIF in the sera of healthy donors and patients with various diseases was determined. GIF was detected in the sera of most healthy individuals and its concentration tended to increase with age. It was also found that the serum GIF levels markedly increased in some patients with rheumatoid arthritis or malignant tumors. Analysis of serum samples by SDS-PAGE and immunoblotting revealed a 55 kDa protein that has both the GIF antigenic determinant and the TCR α chain determinant. A 13 kDa GIF was not detected in the sera. In view of our previous findings on antigen-specific GIF from murine suppressor T cell hybridomas indicating that the 55 kDa GIF is a post-translationaly formed conjugate of a TCR α chain with 13 kDa GIF, we suspect that the 55 kDa GIF detected in human sera is a human homologue of the murine 55 kDa GIF.",

keywords = "Aging, Malignant tumor, Rheumatoid arthritis, T(S) cell",

author = "Kaoru Kasahara and Tatsumi Nakano and Hiroki Takahashi and Yasuyuki Ishii and Kimishige Ishizaka and Kohzoh Imai",

year = "2000",

doi = "10.1093/intimm/12.9.1303",

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AU - Kasahara, Kaoru

AU - Nakano, Tatsumi

AU - Takahashi, Hiroki

AU - Ishii, Yasuyuki

AU - Ishizaka, Kimishige

AU - Imai, Kohzoh

PY - 2000

Y1 - 2000

N2 - An ELISA system for the human glycosylation inhibiting factor (GIF) was established using polyclonal antibodies against highly purified 13 kDa recombinant human GIF, and the concentration of GIF in the sera of healthy donors and patients with various diseases was determined. GIF was detected in the sera of most healthy individuals and its concentration tended to increase with age. It was also found that the serum GIF levels markedly increased in some patients with rheumatoid arthritis or malignant tumors. Analysis of serum samples by SDS-PAGE and immunoblotting revealed a 55 kDa protein that has both the GIF antigenic determinant and the TCR α chain determinant. A 13 kDa GIF was not detected in the sera. In view of our previous findings on antigen-specific GIF from murine suppressor T cell hybridomas indicating that the 55 kDa GIF is a post-translationaly formed conjugate of a TCR α chain with 13 kDa GIF, we suspect that the 55 kDa GIF detected in human sera is a human homologue of the murine 55 kDa GIF.

AB - An ELISA system for the human glycosylation inhibiting factor (GIF) was established using polyclonal antibodies against highly purified 13 kDa recombinant human GIF, and the concentration of GIF in the sera of healthy donors and patients with various diseases was determined. GIF was detected in the sera of most healthy individuals and its concentration tended to increase with age. It was also found that the serum GIF levels markedly increased in some patients with rheumatoid arthritis or malignant tumors. Analysis of serum samples by SDS-PAGE and immunoblotting revealed a 55 kDa protein that has both the GIF antigenic determinant and the TCR α chain determinant. A 13 kDa GIF was not detected in the sera. In view of our previous findings on antigen-specific GIF from murine suppressor T cell hybridomas indicating that the 55 kDa GIF is a post-translationaly formed conjugate of a TCR α chain with 13 kDa GIF, we suspect that the 55 kDa GIF detected in human sera is a human homologue of the murine 55 kDa GIF.

KW - Aging

KW - Malignant tumor

KW - Rheumatoid arthritis

KW - T(S) cell

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SN - 0953-8178

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EP - 1309

JO - International Immunology

JF - International Immunology

IS - 9

ER -

Presence of the 55 kDa glycosylation inhibiting factor in human serum

Abstract

Keywords

ASJC Scopus subject areas

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