Presence of lipids in urinary stones: Results of preliminary studies

Saeed R. Khan, Paula N. Shevock, Raymond L. Hackett

Research output: Contribution to journalArticle

Abstract

The presence of lipids in urinary stones was determined by histochemical and biochemical methods. When crystals of calcium oxalate, made by mixing calcium chloride and potassium oxalate solutions and sections of human calcium oxalate urinary stones, were exposed to osmium vapors, there was no staining of the pure crystals whereas the stone sections were stained. De-paraffinized sections of demineralized calcium oxalate stones showed positive sudanophilia on staining with Sudan black B. Both these experiments indicate the presence of lipids in calcium oxalate stones. Lipids were extracted from uric acid, struvite, and calcium oxalate stones using standard techniques. Phospholipids were separated by one-dimensional thin layer chromatography. All the stones studied contained lipids. In calcium oxalate stones they accounted for 10.15% of the matrix. Calcium oxalate and struvite stones contained more phospholipids than uric acid stones. Cardiolipin, sphingomyelin, phosphatidyl choline, phosphatidyl inositol, phosphatidyl ethanolamine, phosphatidyl serine, and phosphatidyl glycerol were identified in lipid extracts. Demineralization by ethylenediaminetetraacetate (EDTA) treatment increased lipid output from calcium oxalate stones by 15.5%.

Original languageEnglish (US)
Pages (from-to)91-96
Number of pages6
JournalCalcified Tissue International
Volume42
Issue number2
DOIs
StatePublished - Mar 1988
Externally publishedYes

Fingerprint

Calcium Oxalate
Urinary Calculi
Lipids
Uric Acid
Phospholipids
Staining and Labeling
Osmium
Oxalic Acid
Calcium Chloride
Phosphatidylglycerols
Ethanolamine
Cardiolipins
Sphingomyelins
Phosphatidylserines
Thin Layer Chromatography
Phosphatidylinositols
Phosphatidylcholines

Keywords

  • Cellular membrane
  • Crystal nucleation
  • Lipids
  • Phospholipids
  • Stone matrix
  • Urinary stone

ASJC Scopus subject areas

  • Endocrinology
  • Orthopedics and Sports Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Presence of lipids in urinary stones : Results of preliminary studies. / Khan, Saeed R.; Shevock, Paula N.; Hackett, Raymond L.

In: Calcified Tissue International, Vol. 42, No. 2, 03.1988, p. 91-96.

Research output: Contribution to journalArticle

Khan, Saeed R. ; Shevock, Paula N. ; Hackett, Raymond L. / Presence of lipids in urinary stones : Results of preliminary studies. In: Calcified Tissue International. 1988 ; Vol. 42, No. 2. pp. 91-96.
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