Presence of complementary RNA in chicken sarcoma cells and Rous sarcoma virus

Ya D. Shaposhnikov; E. A. Ratovitskii; O. K. Kuznetsov

doi:10.1007/BF00801357

Presence of complementary RNA in chicken sarcoma cells and Rous sarcoma virus

Ya D. Shaposhnikov, E. A. Ratovitskii, O. K. Kuznetsov

Research output: Contribution to journal › Article › peer-review

Abstract

The subcellular localization of nucleotide sequences in Rous chicken sarcoma complementary to RNA of Rous sarcoma virus was investigated by RNA-RNA molecular hybridization. Samples of virion RNA labeled with radioactive iodine (¹²⁵I) were annealed with RNA from different fractions (nuclei, mitochondria, free and membrane-bound polysomes) isolated from Rous chicken sarcoma cells. The formation of RNase-resistant hybrids between virus RNA-¹²⁵I and RNA from mitochondria and membrane-bound polysomes was demonstrated; the relative frequency of occurrence of sequences complementary to virion RNA in the latter, moreover, was 446 times greater than in the former. The role of complementary ribonucleotide sequences is discussed.

Original language	English (US)
Pages (from-to)	356-358
Number of pages	3
Journal	Bulletin of Experimental Biology and Medicine
Volume	85
Issue number	3
DOIs	https://doi.org/10.1007/BF00801357
State	Published - Mar 1978
Externally published	Yes

Keywords

complementation
ribunucleic acids
Rous chicken sarcoma
Rous sarcoma virus
subcellular fractions

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1007/BF00801357

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title = "Presence of complementary RNA in chicken sarcoma cells and Rous sarcoma virus",

abstract = "The subcellular localization of nucleotide sequences in Rous chicken sarcoma complementary to RNA of Rous sarcoma virus was investigated by RNA-RNA molecular hybridization. Samples of virion RNA labeled with radioactive iodine (125I) were annealed with RNA from different fractions (nuclei, mitochondria, free and membrane-bound polysomes) isolated from Rous chicken sarcoma cells. The formation of RNase-resistant hybrids between virus RNA-125I and RNA from mitochondria and membrane-bound polysomes was demonstrated; the relative frequency of occurrence of sequences complementary to virion RNA in the latter, moreover, was 446 times greater than in the former. The role of complementary ribonucleotide sequences is discussed.",

keywords = "complementation, ribunucleic acids, Rous chicken sarcoma, Rous sarcoma virus, subcellular fractions",

author = "Shaposhnikov, {Ya D.} and Ratovitskii, {E. A.} and Kuznetsov, {O. K.}",

year = "1978",

month = mar,

doi = "10.1007/BF00801357",

language = "English (US)",

volume = "85",

pages = "356--358",

journal = "Bulletin of Experimental Biology and Medicine",

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TY - JOUR

T1 - Presence of complementary RNA in chicken sarcoma cells and Rous sarcoma virus

AU - Shaposhnikov, Ya D.

AU - Ratovitskii, E. A.

AU - Kuznetsov, O. K.

PY - 1978/3

Y1 - 1978/3

N2 - The subcellular localization of nucleotide sequences in Rous chicken sarcoma complementary to RNA of Rous sarcoma virus was investigated by RNA-RNA molecular hybridization. Samples of virion RNA labeled with radioactive iodine (125I) were annealed with RNA from different fractions (nuclei, mitochondria, free and membrane-bound polysomes) isolated from Rous chicken sarcoma cells. The formation of RNase-resistant hybrids between virus RNA-125I and RNA from mitochondria and membrane-bound polysomes was demonstrated; the relative frequency of occurrence of sequences complementary to virion RNA in the latter, moreover, was 446 times greater than in the former. The role of complementary ribonucleotide sequences is discussed.

AB - The subcellular localization of nucleotide sequences in Rous chicken sarcoma complementary to RNA of Rous sarcoma virus was investigated by RNA-RNA molecular hybridization. Samples of virion RNA labeled with radioactive iodine (125I) were annealed with RNA from different fractions (nuclei, mitochondria, free and membrane-bound polysomes) isolated from Rous chicken sarcoma cells. The formation of RNase-resistant hybrids between virus RNA-125I and RNA from mitochondria and membrane-bound polysomes was demonstrated; the relative frequency of occurrence of sequences complementary to virion RNA in the latter, moreover, was 446 times greater than in the former. The role of complementary ribonucleotide sequences is discussed.

KW - complementation

KW - ribunucleic acids

KW - Rous chicken sarcoma

KW - Rous sarcoma virus

KW - subcellular fractions

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DO - 10.1007/BF00801357

M3 - Article

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SP - 356

EP - 358

JO - Bulletin of Experimental Biology and Medicine

JF - Bulletin of Experimental Biology and Medicine

IS - 3

ER -

Presence of complementary RNA in chicken sarcoma cells and Rous sarcoma virus

Abstract

Keywords

ASJC Scopus subject areas

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