Presence of complementary RNA in chicken sarcoma cells and Rous sarcoma virus

Ya D. Shaposhnikov, E. A. Ratovitskii, O. K. Kuznetsov

Research output: Contribution to journalArticle

Abstract

The subcellular localization of nucleotide sequences in Rous chicken sarcoma complementary to RNA of Rous sarcoma virus was investigated by RNA-RNA molecular hybridization. Samples of virion RNA labeled with radioactive iodine (125I) were annealed with RNA from different fractions (nuclei, mitochondria, free and membrane-bound polysomes) isolated from Rous chicken sarcoma cells. The formation of RNase-resistant hybrids between virus RNA-125I and RNA from mitochondria and membrane-bound polysomes was demonstrated; the relative frequency of occurrence of sequences complementary to virion RNA in the latter, moreover, was 446 times greater than in the former. The role of complementary ribonucleotide sequences is discussed.

Original languageEnglish (US)
Pages (from-to)356-358
Number of pages3
JournalBulletin of Experimental Biology and Medicine
Volume85
Issue number3
DOIs
StatePublished - Mar 1978
Externally publishedYes

Keywords

  • complementation
  • ribunucleic acids
  • Rous chicken sarcoma
  • Rous sarcoma virus
  • subcellular fractions

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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