Prescription opioids. III. Disposition of oxycodone in oral fluid and blood following controlled single-dose administration

Edward J. Cone, Anne Z. De Priest, Rebecca Heltsley, David L. Black, John M. Mitchell, Charles LoDico, Ron Flegel

Research output: Contribution to journalArticlepeer-review

Abstract

Oxycodone (OC) is recommended to be included as an analyte tested in the proposed Substance Abuse and Mental Health Services Administration (SAMHSA's) Mandatory Guidelines for Federal Workplace Drug Testing Programs using Oral Fluid (OF) Specimens. This study demonstrates the time course of OC and metabolites, noroxycodone (NOC), oxymorphone (OM) and noroxymorphone (NOM), in near-simultaneous paired OF and whole blood (BL) specimens by liquid chromatography-tandem mass spectrometry (LC-MS-MS) (limit of detection 5 1 ng/mL OF, 5 ng/mL BL). A single dose of OC 20 mg controlled-releasewas administered to 12 healthy subjects followed by specimen collections for 52 h. Analyte prevalence was as follows: OF, OC > NOC > OM; and BL, OC > NOC > NOM. OC and NOC were frequently detected within 15-30 min in OF and 30 min to 2 h in BL. NOM and OM appeared between 1.5-5 h post-dose. The mean OF-to-BL (OF:BL) ratios and correlations were 5.4 for OC (r = 0.719) and 1.0 for NOC (r = 0.651). The period of detection for OF exceeded BL by ~2-fold at similar cutoff concentrations. At a 1 ng/mL cutoff for OF, the mean detection time was 34 h for OC and NOC. These data provide new information that should facilitate interpretation of OC test results.

Original languageEnglish (US)
Pages (from-to)192-202
Number of pages11
JournalJournal of analytical toxicology
Volume39
Issue number3
DOIs
StatePublished - Apr 1 2015

ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Chemical Health and Safety

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