Abstract
The regional distribution of forebrain neurons expressing preprocholecystokinin (preproCCK) mRNA was examined using in situ and blot hybridization analysis. We observed a distribution of neurons containing preproCCK mRNA closely parallel to that reported for CCK immunoreactivity, with the exception of the thalamus. In this CCK-immunoreactive cell body-poor structure, we detected relatively abundant RNA:probe hybridization using both our techniques. Control experiments suggested that the hybridizing species in thalamus is authentic preproCCK mRNA. This suggests that there exist forebrain neurons expressing the CCK gene which have gone undetected in immunocytochemical studies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 247-253 |
| Number of pages | 7 |
| Journal | Molecular Brain Research |
| Volume | 4 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1988 |
| Externally published | Yes |
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Keywords
- In situ hybridization
- Preprocholecystokinin
- Regional distribution
- RNA blotting
- Thalamus
ASJC Scopus subject areas
- Molecular Biology
- Cellular and Molecular Neuroscience
Cite this
Preprocholecystokinin mRNA in rat brain : regional expression includes thalamus. / Voigt, Mark M.; Uhl, George R.
In: Molecular Brain Research, Vol. 4, No. 3, 1988, p. 247-253.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Preprocholecystokinin mRNA in rat brain
T2 - regional expression includes thalamus
AU - Voigt, Mark M.
AU - Uhl, George R.
PY - 1988
Y1 - 1988
N2 - The regional distribution of forebrain neurons expressing preprocholecystokinin (preproCCK) mRNA was examined using in situ and blot hybridization analysis. We observed a distribution of neurons containing preproCCK mRNA closely parallel to that reported for CCK immunoreactivity, with the exception of the thalamus. In this CCK-immunoreactive cell body-poor structure, we detected relatively abundant RNA:probe hybridization using both our techniques. Control experiments suggested that the hybridizing species in thalamus is authentic preproCCK mRNA. This suggests that there exist forebrain neurons expressing the CCK gene which have gone undetected in immunocytochemical studies.
AB - The regional distribution of forebrain neurons expressing preprocholecystokinin (preproCCK) mRNA was examined using in situ and blot hybridization analysis. We observed a distribution of neurons containing preproCCK mRNA closely parallel to that reported for CCK immunoreactivity, with the exception of the thalamus. In this CCK-immunoreactive cell body-poor structure, we detected relatively abundant RNA:probe hybridization using both our techniques. Control experiments suggested that the hybridizing species in thalamus is authentic preproCCK mRNA. This suggests that there exist forebrain neurons expressing the CCK gene which have gone undetected in immunocytochemical studies.
KW - In situ hybridization
KW - Preprocholecystokinin
KW - Regional distribution
KW - RNA blotting
KW - Thalamus
UR - http://www.scopus.com/inward/record.url?scp=0023711322&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023711322&partnerID=8YFLogxK
U2 - 10.1016/0169-328X(88)90031-9
DO - 10.1016/0169-328X(88)90031-9
M3 - Article
VL - 4
SP - 247
EP - 253
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 3
ER -