Preprocessing, normalization and integration of the Illumina HumanMethylationEPIC array with minfi

Jean Philippe Fortin, Timothy J. Triche, Kasper D. Hansen

Research output: Contribution to journalArticlepeer-review

Abstract

The minfi package is widely used for analyzing Illumina DNA methylation array data. Here we describe modifications to the minfi package required to support the HumanMethylationEPIC ('EPIC') array from Illumina. We discuss methods for the joint analysis and normalization of data from the HumanMethylation450 ('450k') and EPIC platforms. We introduce the single-sample Noob (ssNoob) method, a normalization procedure suitable for incremental preprocessing of individual methylation arrays and conclude that this method should be used when integrating data from multiple generations of Infinium methylation arrays. We show how to use reference 450k datasets to estimate cell type composition of samples on EPIC arrays. The cumulative effect of these updates is to ensure that minfi provides the tools to best integrate existing and forthcoming Illumina methylation array data.

Original languageEnglish (US)
Pages (from-to)558-560
Number of pages3
JournalBioinformatics
Volume33
Issue number4
DOIs
StatePublished - Feb 15 2017

ASJC Scopus subject areas

  • Statistics and Probability
  • Biochemistry
  • Molecular Biology
  • Computer Science Applications
  • Computational Theory and Mathematics
  • Computational Mathematics

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