Preparing, handling, and applying caged compound solutions for acousto-optical deflector-based patterned uncaging with ultraviolet light

Eugene F. Civillico; Shy Shoham; Daniel H. O'Connor; Dmitry V. Sarkisov; Samuel S.H. Wang

doi:10.1101/pdb.prot070656

Preparing, handling, and applying caged compound solutions for acousto-optical deflector-based patterned uncaging with ultraviolet light

Eugene F. Civillico, Shy Shoham, Daniel H. O'Connor, Dmitry V. Sarkisov, Samuel S.H. Wang

School of Medicine

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The method of patterned photoactivation is a natural fit for the study of neuronal dendritic integration. Photoactivatable molecules that influence a wide range of extracellular and intracellular neurophysiological functions are available. The choice of photosensitive molecules depends on the research question and will influence the design of the experimental apparatus. An acousto-optical deflector (AOD)-based system can be used for rapid ultraviolet (UV) photolysis in arbitrary spatial and temporal patterns. This protocol describes how to prepare caged neurotransmitter compound solutions for use in this system and discusses options for introducing caged compounds into an experimental preparation.

Original language	English (US)
Pages (from-to)	923-925
Number of pages	3
Journal	Cold Spring Harbor Protocols
Volume	7
Issue number	8
DOIs	https://doi.org/10.1101/pdb.prot070656
State	Published - Aug 2012

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1101/pdb.prot070656

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abstract = "The method of patterned photoactivation is a natural fit for the study of neuronal dendritic integration. Photoactivatable molecules that influence a wide range of extracellular and intracellular neurophysiological functions are available. The choice of photosensitive molecules depends on the research question and will influence the design of the experimental apparatus. An acousto-optical deflector (AOD)-based system can be used for rapid ultraviolet (UV) photolysis in arbitrary spatial and temporal patterns. This protocol describes how to prepare caged neurotransmitter compound solutions for use in this system and discusses options for introducing caged compounds into an experimental preparation.",

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AU - Wang, Samuel S.H.

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Preparing, handling, and applying caged compound solutions for acousto-optical deflector-based patterned uncaging with ultraviolet light

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