TY - JOUR
T1 - Preparative and analytical applications of immobilized haemoglobin
AU - Chiancone, E.
AU - Gattoni, M.
AU - Fronticelli, C.
AU - Termine, A.
AU - Silvestri, L.
AU - Sarti, P.
PY - 1995/2/3
Y1 - 1995/2/3
N2 - New applications of haemoglobin immobilized on Sepharose 4B are proposed for the removal of cell-free, potentially toxic haemoglobin from either natural biological specimens or artificial haemoglobin-containing systems (e.g., blood substitutes, haemosomes) to be employed for biomedical purposes. In a model study, an affinity column of immobilized haemoglobin was used to remove free haemoglobin from blood serum in which controlled haemolysis had been induced. The affinity column retains all the free haemoglobin, does not retain the haemoglobin-haptoglobin complex(es) and leaves the composition of the serum samples unaltered. When immobilized met-haemoglobin is used, haem is transferred readily to albumin, with which it forms a complex. This observation on the one hand shows that immobilized oxyhaemoglobin should be preferred for preparative purposes, and on the other opens the way to the characterization of the haem transfer reaction to albumin by means of immobilized met-haemoglobin. This reaction is difficult to study in solution owing to the overlap of the met-haemoglobin and methaemalbumin spectra.
AB - New applications of haemoglobin immobilized on Sepharose 4B are proposed for the removal of cell-free, potentially toxic haemoglobin from either natural biological specimens or artificial haemoglobin-containing systems (e.g., blood substitutes, haemosomes) to be employed for biomedical purposes. In a model study, an affinity column of immobilized haemoglobin was used to remove free haemoglobin from blood serum in which controlled haemolysis had been induced. The affinity column retains all the free haemoglobin, does not retain the haemoglobin-haptoglobin complex(es) and leaves the composition of the serum samples unaltered. When immobilized met-haemoglobin is used, haem is transferred readily to albumin, with which it forms a complex. This observation on the one hand shows that immobilized oxyhaemoglobin should be preferred for preparative purposes, and on the other opens the way to the characterization of the haem transfer reaction to albumin by means of immobilized met-haemoglobin. This reaction is difficult to study in solution owing to the overlap of the met-haemoglobin and methaemalbumin spectra.
UR - http://www.scopus.com/inward/record.url?scp=0028876580&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028876580&partnerID=8YFLogxK
U2 - 10.1016/0378-4347(94)00405-T
DO - 10.1016/0378-4347(94)00405-T
M3 - Article
C2 - 7757245
AN - SCOPUS:0028876580
SN - 0378-4347
VL - 664
SP - 89
EP - 95
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - 1
ER -