Preparation of highly purified 3α- and 3β-hydroxysteroid dehydrogenases from Pseudomonas sp

Mikio Shikita, Paul Talalay

Research output: Contribution to journalArticlepeer-review

Abstract

A method is described for preparing highly purified 3α- and 3β-hydroxysteroid dehydrogenases (EC 1.1.1.50 and EC 1.1.1.145, respectively), essentially uncontaminated with one another, from extracts of a steroid-induced Pseudomonas species. These enzymes are suitable for the microanalysis of 3α-hydroxy-, 3β-hydroxy-, and 3-ketosteroids.

Original languageEnglish (US)
Pages (from-to)286-292
Number of pages7
JournalAnalytical biochemistry
Volume95
Issue number1
DOIs
StatePublished - May 1979

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Preparation of highly purified 3α- and 3β-hydroxysteroid dehydrogenases from Pseudomonas sp'. Together they form a unique fingerprint.

Cite this