TY - JOUR
T1 - Preparation of herbal medicine
T2 - Er-xian decoction and er-xian-containing serum for in vivo and in vitro experiments
AU - Liu, Shufen
AU - Sun, Yueli
AU - Li, Ji
AU - Dong, Jingcheng
AU - Bian, Qin
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (81573992). We thank Emily K. Lo and Kathleen DiNapoli for their language editing assistance.
Publisher Copyright:
© 2017 Journal of Visualized Experiments.
PY - 2017/5/31
Y1 - 2017/5/31
N2 - Traditional herbal medicine, an alternative medicine in the clinical setting, has received increased attention in recent years. Before delivery to the body, an additional extraction procedure is commonly required to release the active constituents from raw herbs. Water decoction is a classical extraction procedure that is still broadly used in the clinical settings. Here, we propose a detailed protocol for er-xian decoction (EXD) in order to apply herbal decoctions to experimental studies. The calculation of an animal-appropriate dose is described, as well as the four main steps of EXD: soaking, water decoction, filtration, and concentration. In addition, serum-containing EXD is introduced to rats as a means of in vitro validation. Here, rats were orally administered EXD for three days. Blood samples were then collected, inactivated, centrifuged, and filtered. The serum, diluted with the culture medium, can be utilized to treat cells or tissues in vitro. For example, EXD was applied to both in vivo and in vitro studies and demonstrated that EXD enhances osteogenesis. This protocol can be used as a reference for the preparation and application of herbal medicines.
AB - Traditional herbal medicine, an alternative medicine in the clinical setting, has received increased attention in recent years. Before delivery to the body, an additional extraction procedure is commonly required to release the active constituents from raw herbs. Water decoction is a classical extraction procedure that is still broadly used in the clinical settings. Here, we propose a detailed protocol for er-xian decoction (EXD) in order to apply herbal decoctions to experimental studies. The calculation of an animal-appropriate dose is described, as well as the four main steps of EXD: soaking, water decoction, filtration, and concentration. In addition, serum-containing EXD is introduced to rats as a means of in vitro validation. Here, rats were orally administered EXD for three days. Blood samples were then collected, inactivated, centrifuged, and filtered. The serum, diluted with the culture medium, can be utilized to treat cells or tissues in vitro. For example, EXD was applied to both in vivo and in vitro studies and demonstrated that EXD enhances osteogenesis. This protocol can be used as a reference for the preparation and application of herbal medicines.
KW - Drug-containing serum
KW - Er-xian decoction
KW - Herbal medicine
KW - In vitro
KW - In vivo
KW - Integrative medicine
KW - Issue 123
KW - Medicine
KW - Serum pharmacology
KW - Traditional Chinese medicine
UR - http://www.scopus.com/inward/record.url?scp=85025164932&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85025164932&partnerID=8YFLogxK
U2 - 10.3791/55654
DO - 10.3791/55654
M3 - Article
C2 - 28605360
AN - SCOPUS:85025164932
SN - 1940-087X
VL - 2017
JO - Journal of Visualized Experiments
JF - Journal of Visualized Experiments
IS - 123
M1 - e55654
ER -