Preparation of fragment libraries for next-generation sequencing on the applied biosystems SOLiD platform

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The primary purpose of this protocol is to prepare genomic DNA libraries that can then be analyzed by massively parallel next-generation sequencing on the Applied Biosystems SOLiD platform. This protocol can be adapted to next-generation sequencing workflows to ultimately generate up to 1 billion 50 bp sequence tags from the ends of each of the DNA molecules in the library in a single next-generation sequencing run.

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationDNa
PublisherAcademic Press Inc.
Pages185-200
Number of pages16
ISBN (Print)9780124186873
DOIs
StatePublished - 2013

Publication series

NameMethods in Enzymology
Volume529
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Yegnasubramanian, S. (2013). Preparation of fragment libraries for next-generation sequencing on the applied biosystems SOLiD platform. In Laboratory Methods in Enzymology: DNa (pp. 185-200). (Methods in Enzymology; Vol. 529). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-418687-3.00015-X