Preparation of fragment libraries for next-generation sequencing on the applied biosystems SOLiD platform

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The primary purpose of this protocol is to prepare genomic DNA libraries that can then be analyzed by massively parallel next-generation sequencing on the Applied Biosystems SOLiD platform. This protocol can be adapted to next-generation sequencing workflows to ultimately generate up to 1 billion 50 bp sequence tags from the ends of each of the DNA molecules in the library in a single next-generation sequencing run.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
Pages185-200
Number of pages16
Volume529
DOIs
StatePublished - 2013

Publication series

NameMethods in Enzymology
Volume529
ISSN (Print)00766879
ISSN (Electronic)15577988

Fingerprint

Genomic Library
Workflow
Gene Library
Libraries
Molecules
DNA
caN protocol

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Preparation of fragment libraries for next-generation sequencing on the applied biosystems SOLiD platform. / Yegnasubramanian, S.

Methods in Enzymology. Vol. 529 2013. p. 185-200 (Methods in Enzymology; Vol. 529).

Research output: Chapter in Book/Report/Conference proceedingChapter

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