@inbook{8b4f67fa04264318aa1539dedb8c9735,
title = "Preparation of filamentous proteins for electron microscopy visualization and reconstruction",
abstract = "Cryo electron microscopy (cryo-EM) has become a mainstream tool for determining the structures of macromolecular complexes at the atomic resolution. It has many advantages over other techniques such as X-ray crystallography and nuclear magnetic resonance (NMR). However, it also entails several challenges, a major one being preparation of an ideal sample. Recent studies have identified that DNA sensors and inflammasomes often assemble into filamentous oligomers, which poses a unique set of challenges in preparing ideal samples for high-resolution reconstruction using cryo-EM. This chapter will discuss how to overcome several major issues in cryo-EM sample preparation including construct design, screening using negative stain (ns) EM, and tips on working with filamentous proteins.",
keywords = "Cryo-EM, Filaments, Fusion tags, Negative stain EM, Sample preparation",
author = "Mariusz Matyszewski and Jungsan Sohn",
note = "Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
doi = "10.1016/bs.mie.2019.06.007",
language = "English (US)",
isbn = "9780128183595",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "167--176",
editor = "Jungsan Sohn",
booktitle = "DNA Sensors and Inflammasomes",
}